Any feedback?
Literature summary for 4.2.3.5 extracted from
- A unique reaction in a common pathway. Mechanism and function of chorismate synthase in the shikimate pathway (1999), Planta, 207, 325-334.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0013 | 0.0022 | O5-(1-carboxyvinyl)-3-phosphoshikimate | - |
Escherichia coli |  |
| 0.0027 | 0.007 | O5-(1-carboxyvinyl)-3-phosphoshikimate | - |
Neurospora crassa | |
| 0.0097 | - |
O5-(1-carboxyvinyl)-3-phosphoshikimate | - |
Saccharomyces cerevisiae | |
| 0.011 | - |
O5-(1-carboxyvinyl)-3-phosphoshikimate | enzyme form CS1 | Solanum lycopersicum | |
| 0.0127 | - |
O5-(1-carboxyvinyl)-3-phosphoshikimate | - |
Staphylococcus aureus | |
| 0.027 | - |
O5-(1-carboxyvinyl)-3-phosphoshikimate | - |
Euglena gracilis | |
| 0.053 | - |
O5-(1-carboxyvinyl)-3-phosphoshikimate | - |
Capnoides sempervirens | |
| 0.08 | - |
O5-(1-carboxyvinyl)-3-phosphoshikimate | enzyme form CS2 | Solanum lycopersicum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 39138 | - |
4 * 39138 | Escherichia coli |
| 40800 | - |
2 * 40800, dimer or tetramer | Saccharomyces cerevisiae |
| 40800 | - |
4 * 40800, dimer or tetramer | Saccharomyces cerevisiae |
| 41605 | - |
2 * 41605, dimer or tetramer, enzyme form CS2 | Solanum lycopersicum |
| 41605 | - |
4 * 41605, dimer or tetramer, enzyme form CS2 | Solanum lycopersicum |
| 41700 | - |
x * 41700 | Euglena gracilis |
| 41771 | - |
2 * 41771 | Capnoides sempervirens |
| 41902 | - |
2 * 41902, dimer or tetramer, enzyme form CS1 | Solanum lycopersicum |
| 41902 | - |
4 * 41902, dimer or tetramer, enzyme form CS1 | Solanum lycopersicum |
| 43026 | - |
4 * 43026 | Staphylococcus aureus |
| 46400 | - |
2 * 46400, dimer or tetramer | Neurospora crassa |
| 46400 | - |
4 * 46400, dimer or tetramer | Neurospora crassa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| O5-(1-carboxyvinyl)-3-phosphoshikimate | Staphylococcus aureus | seventh enzyme in shikimate pathway | chorismate + phosphate | - |
? | |
| O5-(1-carboxyvinyl)-3-phosphoshikimate | Escherichia coli | seventh enzyme in shikimate pathway | chorismate + phosphate | - |
? | |
| O5-(1-carboxyvinyl)-3-phosphoshikimate | Saccharomyces cerevisiae | seventh enzyme in shikimate pathway | chorismate + phosphate | - |
? | |
| O5-(1-carboxyvinyl)-3-phosphoshikimate | Euglena gracilis | seventh enzyme in shikimate pathway | chorismate + phosphate | - |
? | |
| O5-(1-carboxyvinyl)-3-phosphoshikimate | Solanum lycopersicum | seventh enzyme in shikimate pathway | chorismate + phosphate | - |
? | |
| O5-(1-carboxyvinyl)-3-phosphoshikimate | Bacillus subtilis | seventh enzyme in shikimate pathway | chorismate + phosphate | - |
? | |
| O5-(1-carboxyvinyl)-3-phosphoshikimate | Capnoides sempervirens | seventh enzyme in shikimate pathway | chorismate + phosphate | - |
? | |
| O5-(1-carboxyvinyl)-3-phosphoshikimate | Neurospora crassa | seventh enzyme in shikimate pathway | chorismate + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | P31104 | - |
- |
| Capnoides sempervirens | P27793 | - |
- |
| Escherichia coli | - |
- |
- |
| Euglena gracilis | - |
- |
- |
| Neurospora crassa | Q12640 | - |
- |
| Saccharomyces cerevisiae | - |
- |
- |
| Solanum lycopersicum | - |
- |
- |
| Staphylococcus aureus | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| proteolytic modification | the precursor protein has a MW of 46871, enzyme form CS2 | Solanum lycopersicum |
| proteolytic modification | the precursor protein has a MW of 47722 Da | Capnoides sempervirens |
| proteolytic modification | the precursor protein has a MW of 47722, enzyme form CS1 | Solanum lycopersicum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | enzyme has flavin reductase activity | Bacillus subtilis | ? | - |
? | |
| additional information | enzyme shows flavin reductase activity | Saccharomyces cerevisiae | ? | - |
? | |
| additional information | enzyme shows flavin reductase activity | Euglena gracilis | ? | - |
? | |
| additional information | enzyme shows flavin reductase activity | Neurospora crassa | ? | - |
? | |
| additional information | no flavin reductase activity | Staphylococcus aureus | ? | - |
? | |
| additional information | no flavin reductase activity | Escherichia coli | ? | - |
? | |
| additional information | no flavin reductase activity | Solanum lycopersicum | ? | - |
? | |
| additional information | no flavin reductase activity | Capnoides sempervirens | ? | - |
? | |
| O5-(1-carboxyvinyl)-3-phosphoshikimate | - |
Staphylococcus aureus | chorismate + phosphate | - |
? | |
| O5-(1-carboxyvinyl)-3-phosphoshikimate | - |
Escherichia coli | chorismate + phosphate | - |
? | |
| O5-(1-carboxyvinyl)-3-phosphoshikimate | - |
Saccharomyces cerevisiae | chorismate + phosphate | - |
? | |
| O5-(1-carboxyvinyl)-3-phosphoshikimate | - |
Euglena gracilis | chorismate + phosphate | - |
? | |
| O5-(1-carboxyvinyl)-3-phosphoshikimate | - |
Solanum lycopersicum | chorismate + phosphate | - |
? | |
| O5-(1-carboxyvinyl)-3-phosphoshikimate | - |
Bacillus subtilis | chorismate + phosphate | - |
? | |
| O5-(1-carboxyvinyl)-3-phosphoshikimate | - |
Capnoides sempervirens | chorismate + phosphate | - |
? | |
| O5-(1-carboxyvinyl)-3-phosphoshikimate | - |
Neurospora crassa | chorismate + phosphate | - |
? | |
| O5-(1-carboxyvinyl)-3-phosphoshikimate | seventh enzyme in shikimate pathway | Staphylococcus aureus | chorismate + phosphate | - |
? | |
| O5-(1-carboxyvinyl)-3-phosphoshikimate | seventh enzyme in shikimate pathway | Escherichia coli | chorismate + phosphate | - |
? | |
| O5-(1-carboxyvinyl)-3-phosphoshikimate | seventh enzyme in shikimate pathway | Saccharomyces cerevisiae | chorismate + phosphate | - |
? | |
| O5-(1-carboxyvinyl)-3-phosphoshikimate | seventh enzyme in shikimate pathway | Euglena gracilis | chorismate + phosphate | - |
? | |
| O5-(1-carboxyvinyl)-3-phosphoshikimate | seventh enzyme in shikimate pathway | Solanum lycopersicum | chorismate + phosphate | - |
? | |
| O5-(1-carboxyvinyl)-3-phosphoshikimate | seventh enzyme in shikimate pathway | Bacillus subtilis | chorismate + phosphate | - |
? | |
| O5-(1-carboxyvinyl)-3-phosphoshikimate | seventh enzyme in shikimate pathway | Capnoides sempervirens | chorismate + phosphate | - |
? | |
| O5-(1-carboxyvinyl)-3-phosphoshikimate | seventh enzyme in shikimate pathway | Neurospora crassa | chorismate + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 41700 | Euglena gracilis |
| dimer | 2 * 40800, dimer or tetramer | Saccharomyces cerevisiae |
| dimer | 2 * 41605, dimer or tetramer, enzyme form CS2 | Solanum lycopersicum |
| dimer | 2 * 41771 | Capnoides sempervirens |
| dimer | 2 * 41902, dimer or tetramer, enzyme form CS1 | Solanum lycopersicum |
| dimer | 2 * 46400, dimer or tetramer | Neurospora crassa |
| tetramer | 4 * 39138 | Escherichia coli |
| tetramer | 4 * 40800, dimer or tetramer | Saccharomyces cerevisiae |
| tetramer | 4 * 41605, dimer or tetramer, enzyme form CS2 | Solanum lycopersicum |
| tetramer | 4 * 41902, dimer or tetramer, enzyme form CS1 | Solanum lycopersicum |
| tetramer | 4 * 43026 | Staphylococcus aureus |
| tetramer | 4 * 46400, dimer or tetramer | Neurospora crassa |
| trimer | heterotrimer | Bacillus subtilis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CS1 | - |
Solanum lycopersicum |
| CS2 | - |
Solanum lycopersicum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8 | enzyme form CS1 | Solanum lycopersicum |
| 6 | 8 | enzyme form CS2 | Solanum lycopersicum |
| 6.5 | 8.5 | - |
Escherichia coli |
| 7 | 8.5 | - |
Neurospora crassa |
| 8 | - |
- |
Capnoides sempervirens |
| 8.2 | - |
- |
Euglena gracilis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FMNH2 | absolute requirement | Solanum lycopersicum | |
| FMNH2 | absolute requirement, Km: 0.0048 mM | Staphylococcus aureus | |
| FMNH2 | absolute requirement, Km: 0.0125 mM | Bacillus subtilis | |
| FMNH2 | absolute requirement, Km: 37 nM | Capnoides sempervirens | |
| FMNH2 | absolute requirement, Km: 42 nM | Saccharomyces cerevisiae | |
| FMNH2 | absolute requirement, Km: 66 nM | Neurospora crassa | |
| FMNH2 | absolute requirement, Km: 76 nM | Euglena gracilis | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Neurospora crassa | - |
- |
4.9 |
| Capnoides sempervirens | - |
- |
5 |
| Euglena gracilis | - |
- |
5.5 |
| Bacillus subtilis | - |
- |
5.5 |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
