Literature summary for 4.2.3.48 extracted from

Li, F.; Li, W.; Lin, Y.; Pickett, J.; Birkett, M.; Wu, K.; Wang, G.; Zhou, J.
Expression of lima bean terpene synthases in rice enhances recruitment of a beneficial enemy of a major rice pest (2017), Plant Cell Environ., 41, 111-120 .

Cloned(Commentary)

Cloned (Comment)	Organism
gene Pltps3, DNA and amino acid sequence determination and analysis, phylogenetic tree of terpene synthases (TPSs), recombinant expression in Oryza sativa ssp. japonica var. ZH11 plants using transformation method via Agrobacterium tumefaciens strain EHA105, real-time quantitative PCR enzyme expression analysis, transgenic rice expressing Pltps3 emitted significantly more (S)-linalool and (3E)-4,8-dimethylnona-1,3,7-triene (DMNT) than wild-type plants, recombinant expression in Escherichia coli strain BL21(DE3)	Phaseolus lunatus
gene Pltps4, DNA and amino acid sequence determination and analysis, phylogenetic tree of terpene synthases (TPSs), recombinant expression in Oryza sativa ssp. japonica var. ZH11 plants using transformation method via Agrobacterium tumefaciens strain EHA105, real-time quantitative PCR enzyme expression analysis, transgenic rice expressing Pltps4 produces (S)-linalool, (E)-4,8-dimethyl-1,3,7-nonatriene (DMNT) and (E,E)-4,8,12-trimethyl-1,3,7,11-tridecatetraene (TMTT), recombinant expression in Escherichia coli strain BL21(DE3)	Phaseolus lunatus

Cloned (Comment)

Organism

gene Pltps3, DNA and amino acid sequence determination and analysis, phylogenetic tree of terpene synthases (TPSs), recombinant expression in Oryza sativa ssp. japonica var. ZH11 plants using transformation method via Agrobacterium tumefaciens strain EHA105, real-time quantitative PCR enzyme expression analysis, transgenic rice expressing Pltps3 emitted significantly more (S)-linalool and (3E)-4,8-dimethylnona-1,3,7-triene (DMNT) than wild-type plants, recombinant expression in Escherichia coli strain BL21(DE3)

Phaseolus lunatus

gene Pltps4, DNA and amino acid sequence determination and analysis, phylogenetic tree of terpene synthases (TPSs), recombinant expression in Oryza sativa ssp. japonica var. ZH11 plants using transformation method via Agrobacterium tumefaciens strain EHA105, real-time quantitative PCR enzyme expression analysis, transgenic rice expressing Pltps4 produces (S)-linalool, (E)-4,8-dimethyl-1,3,7-nonatriene (DMNT) and (E,E)-4,8,12-trimethyl-1,3,7,11-tridecatetraene (TMTT), recombinant expression in Escherichia coli strain BL21(DE3)

Phaseolus lunatus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.
farnesyl diphosphate + H2O	Phaseolus lunatus	-	(E)-nerolidol + diphosphate	-	?
geranyl diphosphate + H2O	Phaseolus lunatus	-	(3S)-linalool + diphosphate	-	?
geranylgeranyl diphosphate + H2O	Phaseolus lunatus	-	(6E,10E)-geranyllinalool + diphosphate	-	?
additional information	Phaseolus lunatus	enzyme PlTPS3 is able to convert GPP to linalool, and FPP to (E)-nerolidol, but PlTPS3 is unable to convert GGPP to (E,E)-geranyllinalool, no detection of TMTT	?	-	?
additional information	Phaseolus lunatus	enzyme PlTPS4 is able to produce linalool, (E)-nerolidol, and (E,E)-geranyllinalool via GPP, FPP and GGPP, respectively	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Phaseolus lunatus	A0A1W5VLZ8	cv. Sieva	-
Phaseolus lunatus	A0A1W5VM12	cv. Sieva	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme from Escherichia coli strain BL21(DE3)	Phaseolus lunatus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
farnesyl diphosphate + H2O	-	Phaseolus lunatus	(E)-nerolidol + diphosphate	-	?
geranyl diphosphate + H2O	-	Phaseolus lunatus	(3S)-linalool + diphosphate	-	?
geranylgeranyl diphosphate + H2O	-	Phaseolus lunatus	(6E,10E)-geranyllinalool + diphosphate	-	?
additional information	no activity with geranylgeranyl diphosphate	Phaseolus lunatus	?	-	?
additional information	enzyme PlTPS3 is able to convert GPP to linalool, and FPP to (E)-nerolidol, but PlTPS3 is unable to convert GGPP to (E,E)-geranyllinalool, no detection of TMTT	Phaseolus lunatus	?	-	?
additional information	enzyme PlTPS4 is able to produce linalool, (E)-nerolidol, and (E,E)-geranyllinalool via GPP, FPP and GGPP, respectively	Phaseolus lunatus	?	-	?

Synonyms

Synonyms	Comment	Organism
Pltps3	-	Phaseolus lunatus
Pltps4	-	Phaseolus lunatus
TPS3	-	Phaseolus lunatus
TPS4	-	Phaseolus lunatus

Cofactor

Cofactor	Comment	Organism	Structure
NADPH	-	Phaseolus lunatus

Expression

Organism	Comment	Expression
Phaseolus lunatus	treatment with either the elicitor alamethicin or spider mites, Tetranychus cinnabarinus, induces the expression of gene Pltps3	up
Phaseolus lunatus	treatment with either the elicitor alamethicin or spider mites, Tetranychus cinnabarinus, induces the expression of gene Pltps4	up

General Information

General Information	Comment	Organism
evolution	PlTP3 is, from a phylogenetic perspective, an ancestor monolineage member in the TPS-g subfamily	Phaseolus lunatus
physiological function	enzyme Pltps3 is involved in the biosynthesis of homoterpenes (also known as tetranorterpenes), i.e. (S)-linalool (EC 4.3.2.25) and the C11 homoterpene (E)-4,8-dimethyl-1,3,7-nonatriene (DMNT). PlTPS3 can discriminate GGPP from GPP and FPP, and is a specific terpene synthase, TPS, involved in DMNT biosynthesis in Phaseolus lunatus	Phaseolus lunatus
physiological function	enzyme Pltps3 is involved in the biosynthesis of homoterpenes (also known as tetranorterpenes), i.e. (S)-linalool, the C11 homoterpene (E)-4,8-dimethyl-1,3,7-nonatriene (DMNT), and the C16 homoterpene (E,E)-4,8,12-trimethyl-1,3,7,11-tridecatetraene (TMTT), via production of the precursors linalool, (E)-nerolidol, and (E,E)-geranyllinalool from GPP, FPP and GGPP, respectively	Phaseolus lunatus