Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Providencia alcalifaciens |
Crystallization (Comment) | Organism |
---|---|
structure in complex with NAD and with NAD plus chlorogenic acid. Chlorogenic acid occupies the substrate position and coordinates with the metal ion in the substrate-binding pocket | Providencia alcalifaciens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
chlorogenic acid | competitive, inhibits the carbon-oxygen lyase activity of DHQS, and also inhibits the growth of Providencia alcalifaciens, Moraxella catarrhalis, Staphylococcus aureus, and Escherichia coli with MIC values of 60 to 100 microM. In the presence of aromatic amino acids supplied externally, chlorogenic acid does not show the toxic effect | Providencia alcalifaciens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0074 | - |
7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate | pH 7.4, 25°C | Providencia alcalifaciens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | In the DHQS-NAD complex structure, Mg2+ shows octahedral coordination geometry, and Mg2+ coordinates with residues His248 and His265, Glu184 and three water molecules | Providencia alcalifaciens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
80000 | - |
PAGE | Providencia alcalifaciens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Providencia alcalifaciens | X6Q997 | - |
- |
Providencia alcalifaciens F90-2004 | X6Q997 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate | - |
Providencia alcalifaciens | 3-dehydroquinate + phosphate | - |
? | |
7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate | - |
Providencia alcalifaciens F90-2004 | 3-dehydroquinate + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 41000, SDS-PAGE of recombinant His-tagged enzyme | Providencia alcalifaciens |
Synonyms | Comment | Organism |
---|---|---|
aroB | - |
Providencia alcalifaciens |
DHQS | - |
Providencia alcalifaciens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.5 | - |
7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate | pH 7.4, 25°C | Providencia alcalifaciens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | cofactor NAD binds in the cleft region formed between the N- and C-terminal domains of each DHQS monomer, with its nicotinamide ring oriented toward the metal ion | Providencia alcalifaciens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.236 | - |
chlorogenic acid | pH 7.4, 25°C | Providencia alcalifaciens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
203 | - |
7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate | pH 7.4, 25°C | Providencia alcalifaciens |