Any feedback?
Literature summary for 4.2.3.3 extracted from
- Structural basis for the regulatory interaction of the methylglyoxal synthase MgsA with the carbon flux regulator Crh in Bacillus subtilis (2018), J. Biol. Chem., 293, 5781-5792 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Bacillus subtilis |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| sitting drop vapor diffusion method, using 10% (w/v) PEG 4000 | Bacillus subtilis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| Q62R | inactive | Bacillus subtilis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Crh protein | in the absence of preferred carbon sources, the phosphoprotein Crh is present in the nonphosphorylated state and binds to and thereby inhibits the enzyme | Bacillus subtilis |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 105000 | - |
gel filtration | Bacillus subtilis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glycerone phosphate | Bacillus subtilis | - |
2-oxopropanal + phosphate | - |
? |  |
| glycerone phosphate | Bacillus subtilis 168 | - |
2-oxopropanal + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | P42980 | - |
- |
| Bacillus subtilis 168 | P42980 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Strep-Tactin column chromatography and Sepharose S75 gel filtration | Bacillus subtilis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 150 | - |
wild type enzyme, at pH 7.5 and 30°C | Bacillus subtilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glycerone phosphate | - |
Bacillus subtilis | 2-oxopropanal + phosphate | - |
? | |
| glycerone phosphate | - |
Bacillus subtilis 168 | 2-oxopropanal + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homohexamer | - |
Bacillus subtilis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MgsA | - |
Bacillus subtilis |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
