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Literature summary for 4.2.3.3 extracted from

  • Atabakhshi-Kashi, M.; Mohammadi, M.; Mirhassani, R.; Dabirmanesh, B.; Sajedi, R.H.; Khajeh, K.
    An alternative allosteric pathway in thermophilic methylglyoxal synthase (2016), Int. J. Biol. Macromol., 93, 526-533 .
    View publication on PubMed
Search for more literature for 4.2.3.3

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Thermus sp. GH5

Protein Variants

Protein Variants Comment Organism
D100N the mutant shows reduced catalytic efficiency compared to the wild type enzyme Thermus sp. GH5
D100V the mutant shows reduced catalytic efficiency compared to the wild type enzyme Thermus sp. GH5
R80D/D100R the mutant shows increased catalytic efficiency compared to the wild type enzyme Thermus sp. GH5
R80I the mutant shows reduced catalytic efficiency compared to the wild type enzyme Thermus sp. GH5
R80K the mutant shows reduced catalytic efficiency compared to the wild type enzyme Thermus sp. GH5
R80Q the mutant shows reduced catalytic efficiency compared to the wild type enzyme Thermus sp. GH5

Inhibitors

Inhibitors Comment Organism Structure
phosphate allosteric inhibitor Thermus sp. GH5

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.3
-
 glycerone phosphate mutant enzyme D100V, at pH 6.2 and 60°C Thermus sp. GH5
0.5
-
 glycerone phosphate mutant enzyme R80D/D100R, at pH 6.2 and 60°C Thermus sp. GH5
0.8
-
 glycerone phosphate mutant enzyme R8Q, at pH 6.2 and 60°C Thermus sp. GH5
0.8
-
 glycerone phosphate wild type enzyme, at pH 6.2 and 60°C Thermus sp. GH5
0.9
-
 glycerone phosphate mutant enzyme R80K, at pH 6.2 and 60°C Thermus sp. GH5
1
-
 glycerone phosphate mutant enzyme D100N, at pH 6.2 and 60°C Thermus sp. GH5
1.2
-
 glycerone phosphate mutant enzyme R80I, at pH 6.2 and 60°C Thermus sp. GH5

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glycerone phosphate Thermus sp. GH5
-
 2-oxopropanal + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Thermus sp. GH5 B3VH91
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Thermus sp. GH5

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glycerone phosphate
-
 Thermus sp. GH5 2-oxopropanal + phosphate
-
 ?

Subunits

Subunits Comment Organism
homohexamer
-
 Thermus sp. GH5

Synonyms

Synonyms Comment Organism
MGS
-
 Thermus sp. GH5

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70 80 the enzyme shows no loss of activity after 150 min at 70°C, shows about 70% activity after 150 min at 75°C, and is almost inactivated after 60 min at 80°C Thermus sp. GH5

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
8
-
 glycerone phosphate mutant enzyme R8Q, at pH 6.2 and 60°C Thermus sp. GH5
13
-
 glycerone phosphate mutant enzyme D100V, at pH 6.2 and 60°C Thermus sp. GH5
15
-
 glycerone phosphate mutant enzyme R80I, at pH 6.2 and 60°C Thermus sp. GH5
29
-
 glycerone phosphate mutant enzyme D100N, at pH 6.2 and 60°C Thermus sp. GH5
38
-
 glycerone phosphate mutant enzyme R80K, at pH 6.2 and 60°C Thermus sp. GH5
162
-
 glycerone phosphate mutant enzyme R80D/D100R, at pH 6.2 and 60°C Thermus sp. GH5
218
-
 glycerone phosphate wild type enzyme, at pH 6.2 and 60°C Thermus sp. GH5

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
9.8
-
 glycerone phosphate mutant enzyme R8Q, at pH 6.2 and 60°C Thermus sp. GH5
12.5
-
 glycerone phosphate mutant enzyme R80I, at pH 6.2 and 60°C Thermus sp. GH5
30
-
 glycerone phosphate mutant enzyme D100N, at pH 6.2 and 60°C Thermus sp. GH5
42
-
 glycerone phosphate mutant enzyme R80K, at pH 6.2 and 60°C Thermus sp. GH5
43
-
 glycerone phosphate mutant enzyme D100V, at pH 6.2 and 60°C Thermus sp. GH5
272
-
 glycerone phosphate wild type enzyme, at pH 6.2 and 60°C Thermus sp. GH5
324
-
 glycerone phosphate mutant enzyme R80D/D100R, at pH 6.2 and 60°C Thermus sp. GH5