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Literature summary for 4.2.3.3 extracted from

  • Falahati, H.; Pazhang, M.; Zareian, S.; Ghaemi, N.; Rofougaran, R.; Hofer, A.; Rezaie, A.; Khajeh, K.
    Transmitting the allosteric signal in methylglyoxal synthase (2013), Protein Eng. Des. Sel., 26, 445-452.
    View publication on PubMed
Search for more literature for 4.2.3.3

Protein Variants

Protein Variants Comment Organism
G56A significant increase in Hill coefficient, large decrease in turnover number Thermus sp.
G56S significant increase in Hill coefficient, large decrease in turnover number Thermus sp.
additional information mutant protein carrying the C-terminal tail of Escherichia coli enzyme containing an Arg residue shows elevated cooperativity in the presence of phosphate Thermus sp.
R97K mutant has lost its heterotropic cooperativity, exhibits homotropic allostery, displays significantly reduced catalytic efficiency Thermus sp.
S55D mutant has lost its allostery Thermus sp.
S55K increase in sensitivity to phosphate Thermus sp.
V101A mutant has lost its allostery Thermus sp.
V101I mutant has lost its heterotropic cooperativity, exhibits homotropic allostery, displays significantly reduced catalytic efficiency Thermus sp.
V101S mutant has lost its heterotropic cooperativity, exhibits homotropic allostery, displays significantly reduced catalytic efficiency Thermus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.08
-
 dihydroxyacetone phosphate mutant S55K, Hill coefficient 1.3, pH 6.5, 60°C Thermus sp.
0.21
-
 dihydroxyacetone phosphate mutant carrying C-terminus of Escherichia coli enzyme, Hill coefficient 0.87, pH 6.5, 60°C Thermus sp.
0.41
-
 dihydroxyacetone phosphate mutant mutant carrying C-terminus of Escherichia coli enzyme, Hill coefficient 1.99, presence of 1.5 mM phosphate, pH 6.5, 60°C Thermus sp.
0.5
-
 dihydroxyacetone phosphate wild-type, Hill coefficient 1, pH 6.5, 60°C Thermus sp.
1.03
-
 dihydroxyacetone phosphate mutant V101A, Hill coefficient 1, pH 6.5, 60°C Thermus sp.
1.17
-
 dihydroxyacetone phosphate wild-type, Hill coefficient 1.5, presence of 1.5 mM phosphate, pH 6.5, 60°C Thermus sp.
1.25
-
 dihydroxyacetone phosphate mutant G56S, Hill coefficient 2.3, pH 6.5, 60°C Thermus sp.
1.38
-
 dihydroxyacetone phosphate mutant S55K, Hill coefficient 1.3, presence of 1.5 mM phosphate, pH 6.5, 60°C Thermus sp.
1.44
-
 dihydroxyacetone phosphate mutant V101I, Hill coefficient 1.44, pH 6.5, 60°C Thermus sp.
1.65
-
 dihydroxyacetone phosphate mutant V101S, Hill coefficient 1.3, pH 6.5, 60°C Thermus sp.
1.81
-
 dihydroxyacetone phosphate mutant V101A, Hill coefficient 1.45, presence of 1.5 mM phosphate, pH 6.5, 60°C Thermus sp.
2.4
-
 dihydroxyacetone phosphate mutant G56A, Hill coefficient 2, pH 6.5, 60°C Thermus sp.
2.7
-
 dihydroxyacetone phosphate mutant V101I, Hill coefficient 1.4, presence of 1.5 mM phosphate, pH 6.5, 60°C Thermus sp.
2.76
-
 dihydroxyacetone phosphate mutant G56A, Hill coefficient 1.6, presence of 1.5 mM phosphate, pH 6.5, 60°C Thermus sp.
2.76
-
 dihydroxyacetone phosphate mutant S55D, Hill coefficient 1, pH 6.5, 60°C Thermus sp.
2.79
-
 dihydroxyacetone phosphate mutant R97K, Hill coefficient 1.55, pH 6.5, 60°C Thermus sp.
3.27
-
 dihydroxyacetone phosphate mutant S55D, Hill coefficient 1.2, presence of 1.5 mM phosphate, pH 6.5, 60°C Thermus sp.
3.5
-
 dihydroxyacetone phosphate mutant G56S, Hill coefficient 2.8, presence of 1.5 mM phosphate, pH 6.5, 60°C Thermus sp.
5.03
-
 dihydroxyacetone phosphate mutant V101S, Hill coefficient 1.22, presence of 1.5 mM phosphate, pH 6.5, 60°C Thermus sp.
6.3
-
 dihydroxyacetone phosphate mutant R97K, Hill coefficient 1.55, presence of 1.5 mM phosphate, pH 6.5, 60°C Thermus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
14300
-
 2 * 14300, calculated, minor form. The main form is the hexamer Thermus sp.
14300
-
 6 * 14300, calculated, main form plus some dimer Thermus sp.
31000
-
 GEMMA analysis, minor peak Thermus sp.
89000
-
 GEMMA analysis, main peak Thermus sp.

Organism

Organism UniProt Comment Textmining
Thermus sp. B3VH91
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dihydroxyacetone phosphate
-
 Thermus sp. methylglyoxal + phosphate
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 14300, calculated, minor form. The main form is the hexamer Thermus sp.
hexamer 6 * 14300, calculated, main form plus some dimer Thermus sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00003
-
 dihydroxyacetone phosphate mutant G56S, Hill coefficient 2.8, presence of 1.5 mM phosphate, pH 6.5, 60°C Thermus sp.
0.00005
-
 dihydroxyacetone phosphate mutant G56S, Hill coefficient 2.3, pH 6.5, 60°C Thermus sp.
0.0001
-
 dihydroxyacetone phosphate mutant G56A, Hill coefficient 1.6, presence of 1.5 mM phosphate, pH 6.5, 60°C Thermus sp.
0.00013
-
 dihydroxyacetone phosphate mutant G56A, Hill coefficient 2, pH 6.5, 60°C Thermus sp.
0.37
-
 dihydroxyacetone phosphate mutant V101I, Hill coefficient 1.4, presence of 1.5 mM phosphate, pH 6.5, 60°C Thermus sp.
0.55
-
 dihydroxyacetone phosphate mutant S55K, Hill coefficient 1.3, presence of 1.5 mM phosphate, pH 6.5, 60°C Thermus sp.
0.62
-
 dihydroxyacetone phosphate mutant R97K, Hill coefficient 1.55, presence of 1.5 mM phosphate, pH 6.5, 60°C Thermus sp.
0.75
-
 dihydroxyacetone phosphate mutant R97K, Hill coefficient 1.55, pH 6.5, 60°C Thermus sp.
0.75
-
 dihydroxyacetone phosphate mutant V101I, Hill coefficient 1.44, pH 6.5, 60°C Thermus sp.
1
-
 dihydroxyacetone phosphate mutant S55K, Hill coefficient 1.3, pH 6.5, 60°C Thermus sp.
1.1
-
 dihydroxyacetone phosphate mutant V101S, Hill coefficient 1.22, presence of 1.5 mM phosphate, pH 6.5, 60°C Thermus sp.
1.4
-
 dihydroxyacetone phosphate mutant V101S, Hill coefficient 1.3, pH 6.5, 60°C Thermus sp.
76
-
 dihydroxyacetone phosphate mutant mutant carrying C-terminus of Escherichia coli enzyme, Hill coefficient 1.99, presence of 1.5 mM phosphate, pH 6.5, 60°C Thermus sp.
100
-
 dihydroxyacetone phosphate mutant carrying C-terminus of Escherichia coli enzyme, Hill coefficient 0.87, pH 6.5, 60°C Thermus sp.
220
-
 dihydroxyacetone phosphate wild-type, Hill coefficient 1.5, presence of 1.5 mM phosphate, pH 6.5, 60°C Thermus sp.
240
-
 dihydroxyacetone phosphate mutant V101A, Hill coefficient 1.45, presence of 1.5 mM phosphate, pH 6.5, 60°C Thermus sp.
260
-
 dihydroxyacetone phosphate mutant S55D, Hill coefficient 1.2, presence of 1.5 mM phosphate, pH 6.5, 60°C Thermus sp.
300
-
 dihydroxyacetone phosphate mutant S55D, Hill coefficient 1, pH 6.5, 60°C Thermus sp.
327
-
 dihydroxyacetone phosphate wild-type, Hill coefficient 1, pH 6.5, 60°C Thermus sp.
338
-
 dihydroxyacetone phosphate mutant V101A, Hill coefficient 1, pH 6.5, 60°C Thermus sp.

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.00004
-
 dihydroxyacetone phosphate mutant G56A, Hill coefficient 1.6, presence of 1.5 mM phosphate, pH 6.5, 60°C Thermus sp.
0.00004
-
 dihydroxyacetone phosphate mutant G56S, Hill coefficient 2.3, pH 6.5, 60°C Thermus sp.
0.00005
-
 dihydroxyacetone phosphate mutant G56A, Hill coefficient 2, pH 6.5, 60°C Thermus sp.
0.00009
-
 dihydroxyacetone phosphate mutant G56S, Hill coefficient 2.8, presence of 1.5 mM phosphate, pH 6.5, 60°C Thermus sp.
0.1
-
 dihydroxyacetone phosphate mutant R97K, Hill coefficient 1.55, presence of 1.5 mM phosphate, pH 6.5, 60°C Thermus sp.
0.14
-
 dihydroxyacetone phosphate mutant V101I, Hill coefficient 1.4, presence of 1.5 mM phosphate, pH 6.5, 60°C Thermus sp.
0.22
-
 dihydroxyacetone phosphate mutant V101S, Hill coefficient 1.22, presence of 1.5 mM phosphate, pH 6.5, 60°C Thermus sp.
0.27
-
 dihydroxyacetone phosphate mutant R97K, Hill coefficient 1.55, pH 6.5, 60°C Thermus sp.
0.4
-
 dihydroxyacetone phosphate mutant S55K, Hill coefficient 1.3, presence of 1.5 mM phosphate, pH 6.5, 60°C Thermus sp.
0.52
-
 dihydroxyacetone phosphate mutant V101I, Hill coefficient 1.44, pH 6.5, 60°C Thermus sp.
0.86
-
 dihydroxyacetone phosphate mutant V101S, Hill coefficient 1.3, pH 6.5, 60°C Thermus sp.
12
-
 dihydroxyacetone phosphate mutant S55K, Hill coefficient 1.3, pH 6.5, 60°C Thermus sp.
79
-
 dihydroxyacetone phosphate mutant S55D, Hill coefficient 1.2, presence of 1.5 mM phosphate, pH 6.5, 60°C Thermus sp.
110
-
 dihydroxyacetone phosphate mutant S55D, Hill coefficient 1, pH 6.5, 60°C Thermus sp.
130
-
 dihydroxyacetone phosphate mutant V101A, Hill coefficient 1.45, presence of 1.5 mM phosphate, pH 6.5, 60°C Thermus sp.
180
-
 dihydroxyacetone phosphate mutant mutant carrying C-terminus of Escherichia coli enzyme, Hill coefficient 1.99, presence of 1.5 mM phosphate, pH 6.5, 60°C Thermus sp.
190
-
 dihydroxyacetone phosphate wild-type, Hill coefficient 1.5, presence of 1.5 mM phosphate, pH 6.5, 60°C Thermus sp.
330
-
 dihydroxyacetone phosphate mutant V101A, Hill coefficient 1, pH 6.5, 60°C Thermus sp.
490
-
 dihydroxyacetone phosphate mutant carrying C-terminus of Escherichia coli enzyme, Hill coefficient 0.87, pH 6.5, 60°C Thermus sp.
653
-
 dihydroxyacetone phosphate wild-type, Hill coefficient 1, pH 6.5, 60°C Thermus sp.