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Literature summary for 4.2.3.29 extracted from

  • Toyomasu, T.; Miyamoto, K.; Shenton, M.R.; Sakai, A.; Sugawara, C.; Horie, K.; Kawaide, H.; Hasegawa, M.; Chuba, M.; Mitsuhashi, W.; Yamane, H.; Kurata, N.; Okada, K.
    Characterization and evolutionary analysis of ent-kaurene synthase like genes from the wild rice species Oryza rufipogon (2016), Biochem. Biophys. Res. Commun., 480, 402-408 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene KSL10, sequence comparisons and phylogenetic analysis and tree Oryza sativa Japonica Group
gene KSL10i, sequence comparisons and phylogenetic analysis and tree Oryza rufipogon
gene KSL10j, sequence comparisons and phylogenetic analysis and tree Oryza rufipogon

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Oryza rufipogon
Mg2+ required Oryza sativa Japonica Group

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ent-copalyl diphosphate Oryza rufipogon
-
ent-sandaracopimara-8(14),15-diene + diphosphate
-
?
ent-copalyl diphosphate Oryza sativa Japonica Group
-
ent-sandaracopimara-8(14),15-diene + diphosphate
-
?

Organism

Organism UniProt Comment Textmining
Oryza rufipogon A0A1L7NUB5 cv. W106
-
Oryza rufipogon A0A2Z6BAT9 cv. W1943
-
Oryza sativa Japonica Group Q2QQJ5
-
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Oryza rufipogon
-
leaf
-
Oryza sativa Japonica Group
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ent-copalyl diphosphate
-
Oryza rufipogon ent-sandaracopimara-8(14),15-diene + diphosphate
-
?
ent-copalyl diphosphate
-
Oryza sativa Japonica Group ent-sandaracopimara-8(14),15-diene + diphosphate
-
?

Synonyms

Synonyms Comment Organism
ent-kaurene synthase like 10 UniProt Oryza rufipogon
ent-sandaracopimaradiene synthase
-
Oryza rufipogon
ent-sandaracopimaradiene synthase
-
Oryza sativa Japonica Group
KS like 10
-
Oryza sativa Japonica Group
KSL10
-
Oryza sativa Japonica Group
KSL10i
-
Oryza rufipogon
KSL10j
-
Oryza rufipogon
OrKSL10i
-
Oryza rufipogon
OrKSL10j
-
Oryza rufipogon
OsKSL10
-
Oryza sativa Japonica Group

General Information

General Information Comment Organism
evolution enzymes KSLs belong to the type A/class I terpene synthase family Oryza rufipogon
evolution enzymes KSLs belong to the type A/class I terpene synthase family Oryza sativa Japonica Group
metabolism LC-MS/MS analyses show that oryzalexins A and C are not detected in either accession W0106 or W1943. Oryzalexins E and F, which are not detected in the two Or accessions, are also undetectable in Oryza sativa cv. Nipponbare. OrKSL10 and OrKSL10ind cannot usually produce ent-sandaracopimaradiene strongly suggesting that no accumulation of oryzalexins A-F is possible in either of the two Or accessions Oryza rufipogon
additional information recombinant isozymes OrKSL10j (from cv. W106) and OrKSL10ind (from cv. W1943) have similar activity Oryza rufipogon
physiological function OrKSL10j and OrKSL10ind encode ent-sandaracopimaradiene synthase responsible for the biosynthesis of oryzalexins A-F Oryza rufipogon
physiological function OsKSL10 encodes ent-sandaracopimaradiene synthase responsible for the biosynthesis of oryzalexins A-F, but oryzalexins E and F are undetectable in Oryza sativa cv. Nipponbare, they are also undetectable in Oryza rufipogon accessions W106 and W1943 Oryza sativa Japonica Group