Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diethyldicarbonate | 34-59% inhibition at 1.1 mM, dimethylally diphosphate and Mg2+ protect the soluble enzyme, but not the solubilized thylakoid membrane isozyme | Salix discolor | |
diphosphate | 69-100% inhibition | Salix discolor | |
additional information | no inhibition by phosphate | Salix discolor | |
NEM | complete inactivation at 1.1 mM, dimethylally diphosphate and Mg2+ protect the isozyme partially | Salix discolor | |
Phenylglyoxal | 50 mM, complete inactivation, dimethylally diphosphate and Mg2+ protect the isozyme partially | Salix discolor |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | soluble and membrane-bound isozymes | Salix discolor |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | soluble and membrane-bound isozymes | Salix discolor | 9507 | - |
chloroplast stroma | soluble isozyme | Salix discolor | 9570 | - |
soluble | soluble enzyme form | Salix discolor | - |
- |
thylakoid membrane | tightly membrane-bound isozyme with a stromal facing, membrane association via a membrane tail, accessible to trypsin treatment | Salix discolor | 42651 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | dependent on, isozyme activity profiles, optimal at 10-20 mM | Salix discolor |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethylallyl diphosphate | Salix discolor | light- and age-dependent regulation of the thylakoid membrane-bound enzyme, the relation of soluble and membrane-bound isozymes is not affected by light and leaf age, overview | isoprene + diphosphate | isoprene is a volatile product emmitted to the atmosphere | ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salix discolor | - |
soluble and membrane-bound isozymes | - |
Purification (Comment) | Organism |
---|---|
separation of soluble and membrane-bound isozymes, by ammonium sulfate fractionation, chloroplast preparation, PEG precipitation, hydrophobic interaction and ion exchange chromatography, and gel filtration, the thylakoid membrane isozyme is irreversibly solubilized by pH 10.0 treatment, overview | Salix discolor |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Salix discolor | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.001 | - |
purified thylakoid membrane-bound isozyme | Salix discolor |
0.011 | - |
purified soluble isozyme | Salix discolor |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethylallyl diphosphate | - |
Salix discolor | isoprene + diphosphate | i.e. 2-methyl-1,3-butadiene | ir | |
dimethylallyl diphosphate | light- and age-dependent regulation of the thylakoid membrane-bound enzyme, the relation of soluble and membrane-bound isozymes is not affected by light and leaf age, overview | Salix discolor | isoprene + diphosphate | isoprene is a volatile product emmitted to the atmosphere | ir |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
assay at | Salix discolor |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
soluble isozyme | Salix discolor |
10 | - |
thylakoid membrane-bound isozyme | Salix discolor |