Literature summary for 4.2.3.25 extracted from

Sitrit, Y.; Ninio, R.; Bar, E.; Golan, E.; Larkov, O.; Ravid, U.; Lewinsohn, E.
S-Linalool synthase activity in developing fruit of the columnar cactus koubo [Cereus peruvianus (L.) Miller] (2004), Plant Sci., 167, 1257-1262.

Search for more literature for 4.2.3.25

Activating Compound

Activating Compound	Comment	Organism	Structure
DTT	-	Cereus peruvianus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0056	-	Mn2+	cofactor Mn2+, pH 6.6, 30°C	Cereus peruvianus
0.018	-	geranyl diphosphate	pH 6.6, 30°C	Cereus peruvianus
0.06	-	Mg2+	cofactor Mg2+, pH 6.6, 30°C	Cereus peruvianus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	-	Cereus peruvianus
Mn2+	preferred cation	Cereus peruvianus
additional information	divalent cation is required for activity, no activity with Cu2+, Co2+, and Ca2+, in presence of Fe2+ spontaneous enzyme-independent formation of racemic linalool from geranyl diphosphate occurs	Cereus peruvianus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
53000	-	gel filtration	Cereus peruvianus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
geranyl diphosphate + H2O	Cereus peruvianus	enantio-specific reaction, the enzyme plays a pivotal role in production of flavor and aroma of ripe cactus fruits	(3S)-linalool + diphosphate	product identification	?

Organism

Organism	UniProt	Comment	Textmining
Cereus peruvianus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
partially from fruits by anion exchange chromatography	Cereus peruvianus

Source Tissue

Source Tissue	Comment	Organism	Textmining
fruit	ripening, very low levels in green immature fruits, enzyme levels increase during fruit ripening	Cereus peruvianus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
geranyl diphosphate + H2O	enantio-specific reaction, the enzyme plays a pivotal role in production of flavor and aroma of ripe cactus fruits	Cereus peruvianus	(3S)-linalool + diphosphate	product identification	?
geranyl diphosphate + H2O	enantio-specific reaction, 85% optical puritiy of S-linalool	Cereus peruvianus	(3S)-linalool + diphosphate	product identification	?
additional information	spontaneous enzyme-independent formation of racemic linalool from geranyl diphosphate in presence of Fe2+	Cereus peruvianus	?	-	?

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	small scale assay at	Cereus peruvianus
34	-	large scale assay at	Cereus peruvianus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.6	-	small scale assay at	Cereus peruvianus
6.9	-	large scale assay at	Cereus peruvianus

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Release 2023.1 (January 2023)