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Literature summary for 4.2.3.25 extracted from

  • Pichersky, E.; Lewinsohn, E.; Croteau, R.
    Purification and characterization of S-linalool synthase, an enzyme involved in the production of floral scent in Clarkia breweri (1995), Arch. Biochem. Biophys., 316, 803-807.
    View publication on PubMed
Search for more literature for 4.2.3.25

Activating Compound

Activating Compound Comment Organism Structure
DTT required for stability Clarkia breweri

General Stability

General Stability Organism
DTT is required for enzyme stability, in absence of DTT the purified enzyme loses 95% of its activity within 2 h at 4°C Clarkia breweri
hexane and pentane stabilize the enzyme Clarkia breweri

Inhibitors

Inhibitors Comment Organism Structure
additional information no effects by 0.1% w/v Triton X-100 Clarkia breweri
SDS leads to complete loss of activity at about 0.1% Clarkia breweri

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0009
-
 geranyl diphosphate pH 7.4, 20°C Clarkia breweri
0.045
-
 Mn2+ cofactor Mn2+, pH 7.4, 20°C Clarkia breweri
0.33
-
 Mg2+ cofactor Mg2+, pH 7.4, 20°C Clarkia breweri

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ half as effective as Mn2+ Clarkia breweri
Mn2+ absolutely required for activity, preferred divalent cation Clarkia breweri
additional information no activity with Cu2+, Zn2+, Ca2+, Co2+ Clarkia breweri

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
73000
-
 gel filtration Clarkia breweri
79000
-
 1 * 79000, SDS-PAGE Clarkia breweri

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
geranyl diphosphate + H2O Clarkia breweri
-
 (3S)-linalool + diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Clarkia breweri
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 26fold from stigmata of freshly opened flowers by anion exchange and hydroxyapatite chromatography to over 95% purity Clarkia breweri

Reaction

Reaction Comment Organism Reaction ID
geranyl diphosphate + H2O = (3S)-linalool + diphosphate reaction mechanism Clarkia breweri
a

Source Tissue

Source Tissue Comment Organism Textmining
stigma
-
 Clarkia breweri
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0004
-
 purified enzyme Clarkia breweri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-linalyl diphosphate + H2O low activity, 15% of the maximal activity with geranyl diphosphate Clarkia breweri (3S)-linalool + diphosphate
-
 ?
geranyl diphosphate + H2O
-
 Clarkia breweri (3S)-linalool + diphosphate
-
 ?
geranyl diphosphate + H2O enantiospecific reaction Clarkia breweri (3S)-linalool + diphosphate product identification ?
additional information no activity with (R)-linalyl diphosphate Clarkia breweri ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 79000, SDS-PAGE Clarkia breweri

Synonyms

Synonyms Comment Organism
LIS
-
 Clarkia breweri

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20
-
 assay at Clarkia breweri

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 broad optimum Clarkia breweri

pH Range

pH Minimum pH Maximum Comment Organism
6.4 8.4 80% of maximal activity within this range Clarkia breweri