Cloned (Comment) | Organism |
---|---|
recombinant expression of enzyme mutant H212A in Escherichia coli | Fusarium fujikuroi |
recombinant expression of enzyme mutant H302A in Escherichia coli | Physcomitrium patens |
Protein Variants | Comment | Organism |
---|---|---|
H212A | the mutant shows altered substrate specificity compared to wild-type, the single histidine to alanine mutation converts FfCPS/KS into a bifunctional ent-13-epi-manoyl oxide synthase | Fusarium fujikuroi |
H302A | the mutant efficiently produces ent-13-epi-manoyl oxide from geranylgeranyl diphosphate | Physcomitrium patens |
H302A | the mutant shows altered substrate specificity compared to wild-type, the single histidine to alanine mutation converts PpCPS/KS into a bifunctional ent-13-epi-manoyl oxide synthase | Physcomitrium patens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ent-copalyl diphosphate | Arabidopsis thaliana | - |
ent-kaurene + diphosphate | - |
? | |
ent-copalyl diphosphate | Oryza sativa | - |
ent-kaurene + diphosphate | - |
? | |
ent-copalyl diphosphate | Sinorhizobium fredii | - |
ent-kaurene + diphosphate | - |
? | |
ent-copalyl diphosphate | Physcomitrium patens | - |
ent-kaurene + diphosphate | - |
? | |
ent-copalyl diphosphate | Fusarium fujikuroi | - |
ent-kaurene + diphosphate | - |
? | |
ent-copalyl diphosphate | Fusarium fujikuroi CBS 195.34 / IMI 58289 / NRRL A-6831 | - |
ent-kaurene + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Fusarium fujikuroi | S0EA85 | i.e. Gibberella fujikuroi | - |
Fusarium fujikuroi CBS 195.34 / IMI 58289 / NRRL A-6831 | S0EA85 | i.e. Gibberella fujikuroi | - |
Oryza sativa | - |
- |
- |
Physcomitrium patens | - |
- |
- |
Physcomitrium patens | A5A8G0 | - |
- |
Sinorhizobium fredii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme mutant H212A from Escherichia coli | Fusarium fujikuroi |
recombinant enzyme mutant H302A from Escherichia coli | Physcomitrium patens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
8alpha-hydroxy-copalyl diphosphate | produced by recombinant Arabidopsis thaliana ent-copalyl diphosphate synthase mutant H263A | Physcomitrium patens | ent-13-epi-manoyl oxide + diphosphate | - |
? | |
8beta-hydroxy-ent-copalyl diphosphate | - |
Arabidopsis thaliana | ent-13-epi-manoyl oxide + ent-kaurene + diphosphate | - |
? | |
8beta-hydroxy-ent-copalyl diphosphate | - |
Oryza sativa | ent-13-epi-manoyl oxide + ent-kaurene + diphosphate | - |
? | |
ent-copalyl diphosphate | - |
Arabidopsis thaliana | ent-kaurene + diphosphate | - |
? | |
ent-copalyl diphosphate | - |
Oryza sativa | ent-kaurene + diphosphate | - |
? | |
ent-copalyl diphosphate | - |
Sinorhizobium fredii | ent-kaurene + diphosphate | - |
? | |
ent-copalyl diphosphate | - |
Physcomitrium patens | ent-kaurene + diphosphate | - |
? | |
ent-copalyl diphosphate | - |
Fusarium fujikuroi | ent-kaurene + diphosphate | - |
? | |
ent-copalyl diphosphate | - |
Fusarium fujikuroi CBS 195.34 / IMI 58289 / NRRL A-6831 | ent-kaurene + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
bifunctional CPS/KS | - |
Physcomitrium patens |
bifunctional CPS/KS | - |
Fusarium fujikuroi |
copalyl diphosphate/kaurene synthase | - |
Physcomitrium patens |
copalyl diphosphate/kaurene synthase | - |
Fusarium fujikuroi |
FfCPS/KS | - |
Fusarium fujikuroi |
PpCPS/KS | - |
Physcomitrium patens |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme is a class I diterpene cyclase | Physcomitrium patens |
evolution | the enzyme is a class I diterpene cyclase | Fusarium fujikuroi |
additional information | the production of manoyl oxide proceeds from a substrate wherein oxygen is already present in the form of a hydroxyl group added during bicyclization of the general diterpenoid precursor (E,E,E)-geranylgeranyl diphosphate (GGPP) to 8alpha-hydroxy-copalyl diphosphate catalysed by recombinant Arabidopsis thaliana ent-copalyl diphosphate synthase mutant H263A, EC 5.5.1.13. 8alpha-Hydroxy-copalyl diphosphate is then converted to ent-13-epi-manoyl oxide by the modified ent-kaurene synthase function of enzyme FfCPS/KS mutant H212A | Fusarium fujikuroi |
additional information | the production of manoyl oxide proceeds from a substrate wherein oxygen is already present in the form of a hydroxyl group added during bicyclization of the general diterpenoid precursor (E,E,E)-geranylgeranyl diphosphate (GGPP) to 8alpha-hydroxy-copalyl diphosphate catalysed by recombinant Arabidopsis thaliana ent-copalyl diphosphate synthase mutant H263A, EC 5.5.1.13. 8alpha-Hydroxy-copalyl diphosphate is then converted to ent-13-epi-manoyl oxide by the modified ent-kaurene synthase function of enzyme PpCPS/KS mutant H302A | Physcomitrium patens |