Cloned (Comment) | Organism |
---|---|
SmKS DNA and amino acid sequence determination and analysis of full-length enzyme from cDNA, sequence comparisons and phylogenetic analysis and tree, recombinant enzyme expression in Saccharomyces cerevisiae strain BY-T20 | Salvia miltiorrhiza |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a SmKS-SmCPSent fusion enzyme (CPSent is ent-copalyl diphosphate synthase, EC 5.5.1.13), which improves the ent-kaurene precursor supply in transformed Saccharomyces cerevisiae strain SGH5 (BY-T20/pESC-Trp::SmKS-SmCPSent/SmKO + pESC-Leu::SmCPR1) | Salvia miltiorrhiza |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
plastid | the enzyme sequence contains a plastid transit peptide sequence, the first 27 N-terminal amino acids rich in serine and threonine (22%) | Salvia miltiorrhiza | 9536 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ent-copalyl diphosphate | Salvia miltiorrhiza | - |
ent-kaurene + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salvia miltiorrhiza | A0A0U4CDK4 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | the enzyme sequence contains a plastid transit peptide sequence, which is cleaved off for enzyme maturation | Salvia miltiorrhiza |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
hairy root | - |
Salvia miltiorrhiza | - |
root | - |
Salvia miltiorrhiza | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ent-copalyl diphosphate | - |
Salvia miltiorrhiza | ent-kaurene + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
kaurene synthase | - |
Salvia miltiorrhiza |
SmKS | - |
Salvia miltiorrhiza |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme is a monofunctional class I diTPS | Salvia miltiorrhiza |
metabolism | the enzyme catalyzes a step in the gibberellin biosynthetic pathway. The tanshinone biosynthetic pathway is uniquely initiated by a sequential pair of cyclization reactions catalyzed by SmCPS1 and SmKSL1 to produce abietane miltiradiene. Enzyme SmCPSent catalyzes the cyclization of geranylgeranyl diphosphate to ent-copalyl diphosphate (ent-CPP), which is converted to ent-kaurene by ent-kaurene synthase, SmKS. ent-Kaurene oxidase (SmKO) then catalyzes the three-step oxidation of ent-kaurene to ent-kaurenoic acid | Salvia miltiorrhiza |
additional information | the enzyme amino acid sequence contains a DDFFD motif but lacks the DxDD motif, indicating that SmKS is a plant KS protein with monofunctional class I diTPS activity | Salvia miltiorrhiza |