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Literature summary for 4.2.3.19 extracted from

  • Yamamura, Y.; Taguchi, Y.; Ichitani, K.; Umebara, I.; Ohshita, A.; Kurosaki, F.; Lee, J.B.
    Characterization of ent-kaurene synthase and kaurene oxidase involved in gibberellin biosynthesis from Scoparia dulcis (2018), J. Nat. Med., 72, 456-463 .
    View publication on PubMed
Search for more literature for 4.2.3.19

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli C41 (DE3) cells Scoparia dulcis
gene KS, phylogenetic tree, quantitative real-time PCR expression analysis, functional recombinant expression in Escherichia coli strain C41(DE3), GC-MS analysis of diterpene products from hexane extracts of recombinant Escherichia coli harboring the pSdGGeC and pSdKS vectors Scoparia dulcis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ent-copalyl diphosphate Scoparia dulcis
-
 ent-kaurene + diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Scoparia dulcis F6M9X0
-
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Scoparia dulcis
-
additional information relative transcript levels of SdCPS1, SdKS, SdKO1, and SdKO2 are determined in young and mature leaves, upper and lower stems, roots, and lateral roots of Scoparia dulcis Scoparia dulcis
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root
-
 Scoparia dulcis
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root the SdKS gene is mainly expressed in the root and lateral root systems, which are elongating tissues, quantitative real-time PCR expression analysis Scoparia dulcis
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stem
-
 Scoparia dulcis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ent-copalyl diphosphate
-
 Scoparia dulcis ent-kaurene + diphosphate
-
 ?

Synonyms

Synonyms Comment Organism
ent-KS
-
 Scoparia dulcis
SdKS
-
 Scoparia dulcis

General Information

General Information Comment Organism
evolution the enzyme is a class I diterpene synthase Scoparia dulcis
metabolism gibberellins (GAs) are ubiquitous diterpenoids in higher plants, whereas some higher plants produce unique species-specific diterpenoids. In gibberellins biosynthesis, ent-kaurene synthase and ent-kaurene oxidase are key players which catalyze early step(s) of the cyclization and oxidation reactions Scoparia dulcis
metabolism the enzyme is involved in gibberellin biosynthesis Scoparia dulcis
physiological function enzyme SdKS catalyzes a cyclization reaction from ent-copalyl diphosphate to ent-kaurene and is a key enzyme in the gibberellins biosynthesis Scoparia dulcis