Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | 0.001-10 mM, eliminated in presence of 0.1 mM EDTA | Marah macrocarpa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2'-isopropyl-4'(trimethylammonium chloride)-5'-methylphenyl piperidine-1-carboxylate | Amo-1618; strong inhibition of EC 5.5.1.13 activity, very weak inhibition of EC 4.2.3.19 activity | Marah macrocarpa | |
2-(N,N-dimethyl-N-octylammonium bromide)-p-methan-1-ol | strong inhibition of EC 5.5.1.13 activity, very weak inhibition of EC 4.2.3.19 activity | Marah macrocarpa | |
copalyl diphosphate | substrate inhibition above 0.0002 mM, might be an effect of contaminants in substrate preparations | Marah macrocarpa | |
EDTA | - |
Marah macrocarpa | |
N,N-dimethylaminoethyl 2,2-diphenylphenylether | strong inhibition of EC 5.5.1.13 activity, very weak inhibition of EC 4.2.3.19 activity | Marah macrocarpa | |
N-ethylmaleimide | - |
Marah macrocarpa | |
p-hydroxymercuribenzoate | - |
Marah macrocarpa | |
Phosphone D | strong inhibition of EC 5.5.1.13 activity, very weak inhibition of EC 4.2.3.19 activity | Marah macrocarpa | |
Phosphone S | strong inhibition of EC 5.5.1.13 activity, very weak inhibition of EC 4.2.3.19 activity | Marah macrocarpa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0006 | - |
copalyl diphosphate | - |
Marah macrocarpa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | no effect | Marah macrocarpa | |
Co2+ | can partially replace Mg2+ | Marah macrocarpa | |
Mg2+ | - |
Marah macrocarpa | |
Mn2+ | can partially replace Mg2+ | Marah macrocarpa | |
Ni2+ | very low activity | Marah macrocarpa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
gel filtration, molecular weight less than 45000 Da | Marah macrocarpa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ent-copalyl diphosphate | Marah macrocarpa | involved in biosynthesis of gibberellins | ent-kaurene + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Marah macrocarpa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Marah macrocarpa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seed | - |
Marah macrocarpa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.005 | - |
partially purified enzyme | Marah macrocarpa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
copalyl diphosphate | - |
Marah macrocarpa | sandaracopimaradiene + diphosphate | - |
? | |
ent-copalyl diphosphate | - |
Marah macrocarpa | ent-kaurene + diphosphate | - |
? | |
ent-copalyl diphosphate | involved in biosynthesis of gibberellins | Marah macrocarpa | ent-kaurene + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
More | part of bifunctional enzyme catalyzing the two step reaction from trans-geranylgeranyl-diphosphate to ent-kaurene, first step is catalyzed by EC 5.5.1.13 (ent-kaurene synthase A) | Marah macrocarpa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.2 | 7.2 | TES buffer | Marah macrocarpa |
6.9 | - |
imidazole buffer | Marah macrocarpa |
7.2 | 8.2 | Tris buffer | Marah macrocarpa |