Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli as fusion protein with maltose-binding protein, enzyme exclusively shows EC 4.2.3.19 activity | Cucurbita maxima |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
plastid | - |
Cucurbita maxima | 9536 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
81000 | - |
SDS-PAGE, native enzyme | Cucurbita maxima |
89000 | - |
molecular weight of native enzyme predicted from DNA sequence | Cucurbita maxima |
130000 | - |
SDS-PAGE, cloned fusion protein | Cucurbita maxima |
131000 | - |
molecular weight of cloned fusion protein predicted from DNA sequence | Cucurbita maxima |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ent-copalyl diphosphate | Cucurbita maxima | involved in biosynthesis of gibberellins | ent-kaurene + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cucurbita maxima | - |
- |
- |
Purification (Comment) | Organism |
---|---|
cloned enzyme from E. coli and native enzyme from endosperm | Cucurbita maxima |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seed | - |
Cucurbita maxima | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ent-copalyl diphosphate | - |
Cucurbita maxima | ent-kaurene + diphosphate | - |
? | |
ent-copalyl diphosphate | involved in biosynthesis of gibberellins | Cucurbita maxima | ent-kaurene + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
More | part of bifunctional enzyme catalyzing the two step reaction from trans-geranylgeranyl-diphosphate to ent-kaurene, first step is catalyzed by EC 5.5.1.13 (ent-kaurene synthase A) | Cucurbita maxima |