Protein Variants | Comment | Organism |
---|---|---|
D361A | lower turnover with geranylgeranyl diphosphate than wild-type | Abies grandis |
D402A | lower turnover with geranylgeranyl diphosphate than wild-type | Abies grandis |
D402E | lower turnover with geranylgeranyl diphosphate than wild-type | Abies grandis |
D402N | lower turnover with geranylgeranyl diphosphate than wild-type | Abies grandis |
D404A | - |
Abies grandis |
D404E | lower turnover with geranylgeranyl diphosphate than wild-type | Abies grandis |
D404N | lower turnover with geranylgeranyl diphosphate than wild-type | Abies grandis |
D405A | lower turnover with geranylgeranyl diphosphate than wild-type | Abies grandis |
D405E | lower turnover with geranylgeranyl diphosphate than wild-type | Abies grandis |
D405N | lower turnover with geranylgeranyl diphosphate than wild-type | Abies grandis |
E499A | lower turnover with geranylgeranyl diphosphate than wild-type | Abies grandis |
R365A | lower turnover with geranylgeranyl diphosphate than wild-type | Abies grandis |
R411A | lower turnover with geranylgeranyl diphosphate than wild-type | Abies grandis |
R454A | lower turnover with geranylgeranyl diphosphate than wild-type | Abies grandis |
W358A | lower turnover with geranylgeranyl diphosphate than wild-type | Abies grandis |
Y520A | lower turnover with geranylgeranyl diphosphate than wild-type | Abies grandis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
14,15-dihydro-15-aza-geranylgeranyl diphosphate | - |
Abies grandis | |
geranylgeranyl diphosphate | - |
Abies grandis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0002 | - |
copalyl diphosphate | mutants D404N, R411A | Abies grandis | |
0.0003 | - |
copalyl diphosphate | mutants D402A, D402N, D404E | Abies grandis | |
0.0004 | - |
copalyl diphosphate | mutants W358A, D361A, D404A, D405E | Abies grandis | |
0.0005 | - |
copalyl diphosphate | mutants D402E, D405E, R454A, E499A Y520A | Abies grandis | |
0.0007 | - |
copalyl diphosphate | mutants D405N, R365A | Abies grandis | |
132 | - |
copalyl diphosphate | - |
Abies grandis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 10 mM selectively enables the conversion of copalyl diphosphate | Abies grandis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(+)-copalyl-diphosphate | Abies grandis | - |
(-)-abietadiene + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Abies grandis | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(+)-copalyl-diphosphate | - |
Abies grandis | (-)-abietadiene + diphosphate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | - |
copalyl diphosphate | mutants D361A, D402A, D402E, D404N, D404A, D404E, D404N, D405A, D405E, D405N, D621A, E499A, R365A, R411A, R454A, W358A, Y520A | Abies grandis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.7 | - |
with copalyl diphosphate as substrate | Abies grandis |