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Literature summary for 4.2.3.167 extracted from

  • Wang, Q.; Jia, M.; Huh, J.; Muchlinski, A.; Peters, R.; Tholl, D.
    Identification of a dolabellane type diterpene synthase and other root-expressed diterpene synthases in Arabidopsis (2016), Front. Plant Sci., 7, 1761 .
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0063
-
geranylgeranyl diphosphate pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
Arabidopsis thaliana 9507
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
geranylgeranyl diphosphate + H2O Arabidopsis thaliana
-
(3E,7E)-dolabella-3,7-dien-18-ol + diphosphate
-
?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana A0A178U9Y5 bifunctional dolabella-3,7-dien-18-ol synthase/dolathalia-3,7,11-triene synthase, reactions of EC 4.2.3.167 and EC 4.2.3.168, respectively
-

Source Tissue

Source Tissue Comment Organism Textmining
flower ecotype Cvi Arabidopsis thaliana
-
leaf ecotype Cvi Arabidopsis thaliana
-
additional information TPS20 compounds occur in all tissues of ecotype Cvi but are absent in the Col ecotype because of deletion and substitution mutations in the Col TPS20 sequence Arabidopsis thaliana
-
root ecotype Cvi Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
geranylgeranyl diphosphate + H2O
-
Arabidopsis thaliana (3E,7E)-dolabella-3,7-dien-18-ol + diphosphate
-
?
additional information reaction presumably proceeds via dephosphorylation of geranylgeranyl diphosphate and the formation of a dolabelanyl carbocation, which is then converted to dolathaliatriene by a Wagner-Meerwein rearrangement, reaction of EC 4.2.3.168, and deprotonation or directly to dolabelladienol by quenching with water Arabidopsis thaliana ?
-
?

Synonyms

Synonyms Comment Organism
At5g48110
-
Arabidopsis thaliana
TPS20
-
Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.83
-
geranylgeranyl diphosphate pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana

General Information

General Information Comment Organism
physiological function the primary TPS20 diterpene products retard the growth of the root rot pathogen Pythium irregulare but only at concentrations exceeding those in planta Arabidopsis thaliana

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
130
-
geranylgeranyl diphosphate pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana