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Literature summary for 4.2.3.16 extracted from

  • Rajaonarivony, J.I.M.; Gershenzon, J.; Croteau, R.
    Characterization and mechanism of (4S)-limonene synthase, a monoterpene cyclase from the glandular trichomes of peppermint (Mentha x piperita) (1992), Arch. Biochem. Biophys., 296, 49-57.
    View publication on PubMed
Search for more literature for 4.2.3.16

Activating Compound

Activating Compound Comment Organism Structure
Sodium phosphate stimulating at low concentrations Mentha sp.

General Stability

General Stability Organism
unstable in crude secretory cell extract, stable after DEAE-cellulose chromatography Mentha sp.

Inhibitors

Inhibitors Comment Organism Structure
benzyl bromide
-
 Mentha sp.
diethyl dicarbonate
-
 Mentha sp.
linalyl sulfonium ion
-
 Mentha sp.
N-ethylmaleimide reversible by cysteine treatment Mentha sp.
p-hydroxymercuribenzoate pretreatment with geranyl diphosphate and MnCl2 prevents inhibition Mentha sp.
Sodium diphosphate Ki: 1 mM Mentha sp.
Sodium phosphate Ki: 30 mM Mentha sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.001
-
 neryl diphosphate
-
 Mentha sp.
0.0018
-
 geranyl diphosphate
-
 Mentha sp.
0.0019
-
 (3R)-linalyl diphosphate
-
 Mentha sp.
0.0023
-
 (3S)-linalyl diphosphate
-
 Mentha sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ Mn2+ preferred Mentha sp.
Mn2+ required, optimum activity with 2 mM, inhibitory at higher concentrations Mentha sp.
additional information Ca2+, Cd2+, Co2+, Cu2+, Fe2+, Ni2+, Zn2+ activated less than 5% Mentha sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
geranyl diphosphate Mentha sp.
-
 (-)-(4S)-limonene + diphosphate small amounts of (-)-alpha-pinene, (-)-beta-pinene and myrcene as side products ?

Organism

Organism UniProt Comment Textmining
Mentha sp.
-
 M. x piperita
-

Purification (Commentary)

Purification (Comment) Organism
partial Mentha sp.

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Mentha sp.
-

Storage Stability

Storage Stability Organism
4°C, stable for 1 week without loss of activity after DEAE-cellulose chromatography Mentha sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(3R)-linalyl diphosphate
-
 Mentha sp. ?
-
 ?
(3S)-linalyl diphosphate
-
 Mentha sp. ?
-
 ?
geranyl diphosphate
-
 Mentha sp. (-)-(4S)-limonene + diphosphate small amounts of (-)-alpha-pinene, (-)-beta-pinene and myrcene as side products ?
neryl diphosphate
-
 Mentha sp. ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.7
-
-
 Mentha sp.

pH Range

pH Minimum pH Maximum Comment Organism
6.1 7.2 half maximum activity at both pH Mentha sp.

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1
-
 Sodium diphosphate
-
 Mentha sp.
30
-
 Sodium phosphate
-
 Mentha sp.