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Literature summary for 4.2.3.150 extracted from

  • Rinkel, J.; Lauterbach, L.; Rabe, P.; Dickschat, J.
    Two diterpene synthases for spiroalbatene and cembrene A from allokutzneria albata (2018), Angew. Chem. Int. Ed. Engl., 57, 3238-3241 .
    View publication on PubMed
Search for more literature for 4.2.3.150

Cloned(Commentary)

Cloned (Comment) Organism
phylogenetic tree, recombinant expression of wild-type and mutant enzymes in Escherichia coli Allokutzneria albata

Protein Variants

Protein Variants Comment Organism
D212E site-directed mutagenesis, the mutation results in strongly decreased activity with Mg2+ and Mn2+ Allokutzneria albata

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ activates Allokutzneria albata
Mn2+ activates Allokutzneria albata

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
geranylgeranyl diphosphate Allokutzneria albata
-
 (S)-(+)-cembrene A + diphosphate
-
 ?
geranylgeranyl diphosphate Allokutzneria albata DSM 44149
-
 (S)-(+)-cembrene A + diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Allokutzneria albata
-
 isolated from tropical soil
-
Allokutzneria albata DSM 44149
-
 isolated from tropical soil
-

Reaction

Reaction Comment Organism Reaction ID
geranylgeranyl diphosphate = (R)-cembrene A + diphosphate the cyclization mechanism from geranylgeranyl diphosphate to cembrene A requires a 1,14-cyclization to the ent-cembranyl cation and deprotonation of one of the geminal methyl groups, reaction mechanism, overview Allokutzneria albata
a

Source Tissue

Source Tissue Comment Organism Textmining
additional information the enzyme seems to be not expressed in laboratory cultures Allokutzneria albata
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
geranylgeranyl diphosphate
-
 Allokutzneria albata (S)-(+)-cembrene A + diphosphate
-
 ?
geranylgeranyl diphosphate
-
 Allokutzneria albata DSM 44149 (S)-(+)-cembrene A + diphosphate
-
 ?
additional information product identification by NMR spectroscopic analysis. Development of a method that uses the stereoselectively deuterated precursors (S)- and (R)-(1-13C,1-2H)farnesyl diphosphate and (S)- and (R)-(1-13C,1-2H)geranyl diphosphate to determine the absolute configurations of terpenes Allokutzneria albata ?
-
 ?
additional information product identification by NMR spectroscopic analysis. Development of a method that uses the stereoselectively deuterated precursors (S)- and (R)-(1-13C,1-2H)farnesyl diphosphate and (S)- and (R)-(1-13C,1-2H)geranyl diphosphate to determine the absolute configurations of terpenes Allokutzneria albata DSM 44149 ?
-
 ?

Synonyms

Synonyms Comment Organism
CAS
-
 Allokutzneria albata