Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.2.3.149 extracted from

  • Meguro, A.; Tomita, T.; Nishiyama, M.; Kuzuyama, T.
    Identification and characterization of bacterial diterpene cyclases that synthesize the cembrane skeleton (2013), ChemBioChem, 14, 316-321.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.3.149

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Streptomyces sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0421
-
 geranylgeranyl diphosphate pH 7.5, 30°C Streptomyces sp.
0.0937
-
 geranylgeranyl diphosphate pH 7.5, 30°C Streptomyces sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Streptomyces sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
 2 * 38000, SDS-PAGE Streptomyces sp.
42000
-
 2 * 42000, SDS-PAGE Streptomyces sp.
69000
-
 gel filtration Streptomyces sp.
95000
-
 gel filtration Streptomyces sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
geranylgeranyl diphosphate Streptomyces sp.
-
 (R)-nephthenol + diphosphate
-
 ?
geranylgeranyl diphosphate Streptomyces sp. SANK 60404
-
 (R)-nephthenol + diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces sp. M1V9Q0
-
-
Streptomyces sp. M1VDX3
-
-
Streptomyces sp. SANK 60404 M1V9Q0
-
-
Streptomyces sp. SANK 60404 M1VDX3
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Streptomyces sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
geranylgeranyl diphosphate
-
 Streptomyces sp. (R)-nephthenol + diphosphate
-
 ?
geranylgeranyl diphosphate the bifunctional enzyme also produces cembrene C (see EC 4.2.3.148, cembrene C synthase) Streptomyces sp. (R)-nephthenol + diphosphate (R)-nephthenol i.e. 2-[(1R,3E,7E,11E)-4,8,12-trimethyltetradeca-3,7,11-trien-1-yl]propan-2-ol ?
geranylgeranyl diphosphate the enzyme also produces (R)-cembrene A and (1S,4E,8E,12E)-2,2,5,9,13-pentamethylcyclopentadeca-4,8,12-trien-1-ol (see EC 4.2.3.150, cembrene A synthase, and EC 4.2.3.151, pentamethylcyclopentadecatrienol synthase) Streptomyces sp. (R)-nephthenol + diphosphate (R)-nephthenol i.e. 2-[(1R,3E,7E,11E)-4,8,12-trimethyltetradeca-3,7,11-trien-1-yl]propan-2-ol ?
geranylgeranyl diphosphate
-
 Streptomyces sp. SANK 60404 (R)-nephthenol + diphosphate
-
 ?
geranylgeranyl diphosphate the bifunctional enzyme also produces cembrene C (see EC 4.2.3.148, cembrene C synthase) Streptomyces sp. SANK 60404 (R)-nephthenol + diphosphate (R)-nephthenol i.e. 2-[(1R,3E,7E,11E)-4,8,12-trimethyltetradeca-3,7,11-trien-1-yl]propan-2-ol ?
geranylgeranyl diphosphate the enzyme also produces (R)-cembrene A and (1S,4E,8E,12E)-2,2,5,9,13-pentamethylcyclopentadeca-4,8,12-trien-1-ol (see EC 4.2.3.150, cembrene A synthase, and EC 4.2.3.151, pentamethylcyclopentadecatrienol synthase) Streptomyces sp. SANK 60404 (R)-nephthenol + diphosphate (R)-nephthenol i.e. 2-[(1R,3E,7E,11E)-4,8,12-trimethyltetradeca-3,7,11-trien-1-yl]propan-2-ol ?

Subunits

Subunits Comment Organism
homodimer 2 * 38000, SDS-PAGE Streptomyces sp.
homodimer 2 * 42000, SDS-PAGE Streptomyces sp.

Synonyms

Synonyms Comment Organism
DtcycA gene name. Two isozymes with this activity are isolated from the bacterium Streptomyces sp. SANK 60404. The enzyme encoded by the DtcycA gene also produces cembrene C (see EC 4.2.3.148, cembrene C synthase), while the enzyme encoded by the DtcycB gene also produces (R)-cembrene A and (1S,4E,8E,12E)-2,2,5,9,13-pentamethylcyclopentadeca-4,8,12-trien-1-ol (see EC 4.2.3.150, cembrene A synthase, and EC 4.2.3.151 (pentamethylcyclopentadecatrienol synthase)) Streptomyces sp.
DtcycB gene name. The trifunctional enzyme also produces (R)-cembrene A and (1S,4E,8E,12E)-2,2,5,9,13-pentamethylcyclopentadeca-4,8,12-trien-1-ol (see EC 4.2.3.150, cembrene A synthase, and EC 4.2.3.151 (pentamethylcyclopentadecatrienol synthase)) Streptomyces sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Streptomyces sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.022
-
 geranylgeranyl diphosphate pH 7.5, 30°C Streptomyces sp.
0.047
-
 geranylgeranyl diphosphate pH 7.5, 30°C Streptomyces sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay Streptomyces sp.