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Literature summary for 4.2.3.13 extracted from

  • Benedict, C.R.; Lu, J.L.; Pettigrew, D.W.; Liu, J.; Stipanovic, R.D.; Williams, H.J.
    The cyclization of farnesyl diphosphate and nerolidyl diphosphate by a purified recombinant delta-cadinene synthase (2001), Plant Physiol., 125, 1754-1765.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.3.13

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli of CDN1-C1 Gossypium arboreum

Crystallization (Commentary)

Crystallization (Comment) Organism
modeled from amino acid sequence of CDN1-C1, in SWISS-MODEL automated homology modeling server Gossypium arboreum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00065
-
 nerolidyl diphosphate pH 7.5, 30ºC, recombinant CDN1-C1 enzyme Gossypium arboreum
0.00605
-
 2-trans,6-trans-farnesyl diphosphate pH 7.5, 30ºC, recombinant CDN1-C1 enzyme Gossypium arboreum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-trans,6-trans-farnesyl diphosphate Gossypium arboreum
-
 (+)-delta-cadinene + diphosphate
-
 ?
2-trans,6-trans-farnesyl diphosphate Gossypium barbadense the first step enzyme in the conversion of farnesyl diphosphate to sesquiterpene phytoalexins. The reaction is induced by the infection of cotton with Verticillium dahliae (+)-delta-cadinene + diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Gossypium arboreum Q39761 tree cotton
-
Gossypium barbadense
-
 sea island cotton
-

Purification (Commentary)

Purification (Comment) Organism
affinity chromatography, chromatography on glutathione-Sepharose-4B column and anion-exchange column Gossypium arboreum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-trans,6-trans-farnesyl diphosphate
-
 Gossypium barbadense (+)-delta-cadinene + diphosphate
-
 ?
2-trans,6-trans-farnesyl diphosphate
-
 Gossypium arboreum (+)-delta-cadinene + diphosphate
-
 ?
2-trans,6-trans-farnesyl diphosphate the first step enzyme in the conversion of farnesyl diphosphate to sesquiterpene phytoalexins. The reaction is induced by the infection of cotton with Verticillium dahliae Gossypium barbadense (+)-delta-cadinene + diphosphate
-
 ?
nerolidyl diphosphate
-
 Gossypium arboreum (+)-delta-cadinene + alpha-bisabolol + beta-bisabolene + beta-farnesene + ?
-
 ?