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Literature summary for 4.2.3.12 extracted from

  • Seo, K.H.; Supanga, S.; Kim, H.L.; Park, Y.S.; Jeon, C.O.; Lee, K.H.
    Purification, crystallization and preliminary crystallographic analysis of a 6-pyruvoyltetrahydropterin synthase homologue from Escherichia coli (2008), Acta Crystallogr. Sect. F, 64, 105-107.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli as a His-tagged fusion protein Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
ePTPS is crystallized using the hanging-drop vapour-diffusion method. Hexagonal- and rectangular shaped crystals are obtained. Diffraction data are collected from the hexagonal and rectangular crystals to 3.0 and 2.3 A resolution, respectively. The hexagonal plate-shaped crystals belonged to space group P321, with unit-cell parameters a = b = 112.59, c = 68.82 A , and contained two molecules in the asymmetric unit. The rectangular crystals belonged to space group I222, with unit-cell parameters a = 112.76, b = 117.66, c = 153.57 A, and contain six molecules in the asymmetric unit Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
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Purification (Commentary)

Purification (Comment) Organism
using Ni-NTA chromatography Escherichia coli

Synonyms

Synonyms Comment Organism
6-pyruvoyltetrahydropterin synthase
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Escherichia coli
ePTPS
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Escherichia coli