Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli as a His-tagged fusion protein | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
ePTPS is crystallized using the hanging-drop vapour-diffusion method. Hexagonal- and rectangular shaped crystals are obtained. Diffraction data are collected from the hexagonal and rectangular crystals to 3.0 and 2.3 A resolution, respectively. The hexagonal plate-shaped crystals belonged to space group P321, with unit-cell parameters a = b = 112.59, c = 68.82 A , and contained two molecules in the asymmetric unit. The rectangular crystals belonged to space group I222, with unit-cell parameters a = 112.76, b = 117.66, c = 153.57 A, and contain six molecules in the asymmetric unit | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using Ni-NTA chromatography | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
6-pyruvoyltetrahydropterin synthase | - |
Escherichia coli |
ePTPS | - |
Escherichia coli |