KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
6-(L-erythro-1,2-dihydroxypropyl 3-triphosphate)-7,8-dihydropterin | - |
Drosophila melanogaster | |
0.1 | - |
7,8-dihydroneopterin triphosphate | pH 7.5, 37ºC | Drosophila melanogaster |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37500 | - |
x * 37500, SDS-PAGE | Drosophila melanogaster |
37500 | - |
2 * 37500, also evidence for higher multimeric forms, SDS-PAGE | Drosophila melanogaster |
83000 | - |
gel filtration of active enzyme seems to have larger molecular mass forms | Drosophila melanogaster |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
7,8-dihydroneopterin triphosphate | Drosophila melanogaster | assay at | 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate | - |
? | |
additional information | Drosophila melanogaster | the sequence of reaction steps include redox transfer between atoms N5, C6 and C1' and unusual triphosphate elimination at the C2'-C3' bond in the side chain | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Drosophila melanogaster | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, Affigel Blue chromatography, DEAE-Bio-gel chromatography, Affigel Blue chromatography, SDS-PAGE | Drosophila melanogaster |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
head | - |
Drosophila melanogaster | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0632 | - |
- |
Drosophila melanogaster |
Storage Stability | Organism |
---|---|
-20°C, PIPES 50 mM, stable for 1 year | Drosophila melanogaster |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
7,8-dihydroneopterin triphosphate | - |
Drosophila melanogaster | 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate | - |
? | |
7,8-dihydroneopterin triphosphate | assay at | Drosophila melanogaster | 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate | - |
? | |
additional information | the sequence of reaction steps include redox transfer between atoms N5, C6 and C1' and unusual triphosphate elimination at the C2'-C3' bond in the side chain | Drosophila melanogaster | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 37500, SDS-PAGE | Drosophila melanogaster |
dimer | 2 * 37500, also evidence for higher multimeric forms, SDS-PAGE | Drosophila melanogaster |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Drosophila melanogaster |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Drosophila melanogaster |