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Literature summary for 4.2.3.12 extracted from

  • Park, Y.S.; Kim, J.H.; Jacobson, K.B.; Yim, J.J.
    Purification and characterization of 6-pyruvoyl-tetrahydropterin synthase from Drosophila melanogaster (1990), Biochim. Biophys. Acta, 1038, 186-194.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
6-(L-erythro-1,2-dihydroxypropyl 3-triphosphate)-7,8-dihydropterin
-
Drosophila melanogaster
0.1
-
7,8-dihydroneopterin triphosphate pH 7.5, 37ºC Drosophila melanogaster

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37500
-
x * 37500, SDS-PAGE Drosophila melanogaster
37500
-
2 * 37500, also evidence for higher multimeric forms, SDS-PAGE Drosophila melanogaster
83000
-
gel filtration of active enzyme seems to have larger molecular mass forms Drosophila melanogaster

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7,8-dihydroneopterin triphosphate Drosophila melanogaster assay at 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate
-
?
additional information Drosophila melanogaster the sequence of reaction steps include redox transfer between atoms N5, C6 and C1' and unusual triphosphate elimination at the C2'-C3' bond in the side chain ?
-
?

Organism

Organism UniProt Comment Textmining
Drosophila melanogaster
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, Affigel Blue chromatography, DEAE-Bio-gel chromatography, Affigel Blue chromatography, SDS-PAGE Drosophila melanogaster

Source Tissue

Source Tissue Comment Organism Textmining
head
-
Drosophila melanogaster
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0632
-
-
Drosophila melanogaster

Storage Stability

Storage Stability Organism
-20°C, PIPES 50 mM, stable for 1 year Drosophila melanogaster

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7,8-dihydroneopterin triphosphate
-
Drosophila melanogaster 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate
-
?
7,8-dihydroneopterin triphosphate assay at Drosophila melanogaster 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate
-
?
additional information the sequence of reaction steps include redox transfer between atoms N5, C6 and C1' and unusual triphosphate elimination at the C2'-C3' bond in the side chain Drosophila melanogaster ?
-
?

Subunits

Subunits Comment Organism
? x * 37500, SDS-PAGE Drosophila melanogaster
dimer 2 * 37500, also evidence for higher multimeric forms, SDS-PAGE Drosophila melanogaster

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Drosophila melanogaster

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Drosophila melanogaster