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Literature summary for 4.2.3.12 extracted from
- Enzymatic properties of 6-pyruvoyl tetrahydropterin synthase purified from fat bodies of silkworm larvae (1993), Adv. Exp. Med. Biol., 338, 191-194.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | the decrease of this enzyme is the most frequent cause of atypical phenylketonuria | Bombyx mori |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| monoiodoacetate | - |
Bombyx mori |  |
| NEM | - |
Bombyx mori | |
| PCMB | - |
Bombyx mori | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0128 | - |
7,8-dihydroneopterin triphosphate | pH 8.5, 37ºC, 1 mM Mg2+ | Bombyx mori | |
| 0.0169 | - |
7,8-dihydroneopterin triphosphate | pH 8.5 37ºC, 5 mM Mg2+ | Bombyx mori | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | stimulatory effect, 5 mM greatly accelerates activity | Bombyx mori | |
| Ca2+ | stimulatory effect and additive effect at Mg2+, 1 mM | Bombyx mori | |
| Mg2+ | stimulates, Km of 0.2 mM and 2 mM at pH 8.5 and 37ºC | Bombyx mori | |
| Mn2+ | stimulatory effect but additional competitive or inhibitory effect | Bombyx mori | |
| additional information | Cu2+ has no effect on activity | Bombyx mori | |
| Ni2+ | no effect on enzyme activity | Bombyx mori | |
| Sr2+ | 5 mM greatly accelerates activity | Bombyx mori | |
| Zn2+ | no effect on enzyme activity | Bombyx mori | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 18500 | - |
4 * 18500, SDS-PAGE | Bombyx mori |
| 72000 | - |
native PAGE | Bombyx mori |
| 85000 | 87000 | gel filtration | Bombyx mori |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bombyx mori | - |
silkworm | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, hydroxyapatite column, gel filtration | Bombyx mori |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| fat body | larvae | Bombyx mori | - |
| larva | fat bodies | Bombyx mori | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 7,8-dihydroneopterin triphosphate | - |
Bombyx mori | 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 18500, SDS-PAGE | Bombyx mori |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Bombyx mori |
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