Literature summary for 4.2.3.1 extracted from

Graham, D.E.; Taylor, S.M.; Wolf, R.Z.; Namboori, S.C.
Convergent evolution of coenzyme M biosynthesis in the Methanosarcinales: cysteate synthase evolved from an ancestral threonine synthase (2009), Biochem. J., 424, 467-478.

Search for more literature for 4.2.3.1

Cloned(Commentary)

Cloned (Comment)	Organism
His-tagged version expressed in Escherichia coli BL21(DE3)	Methanosarcina acetivorans C2A

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
48000	-	3 * 48000 (calculated 49100), SDS-PAGE, gelfiltration	Methanosarcina acetivorans C2A
135000	-	gel filtration	Methanosarcina acetivorans C2A

Organism

Organism	UniProt	Comment	Textmining
Methanosarcina acetivorans C2A	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni2+-affinity chromatography	Methanosarcina acetivorans C2A

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
1.6	-	-	Methanosarcina acetivorans C2A

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
O-phospho-L-homoserine + H2O	-	Methanosarcina acetivorans C2A	L-threonine + phosphate	-	?

Subunits

Subunits	Comment	Organism
homotrimer	3 * 48000 (calculated 49100), SDS-PAGE, gelfiltration	Methanosarcina acetivorans C2A

Synonyms

Synonyms	Comment	Organism
threonine synthase (gene MA1610)	-	Methanosarcina acetivorans C2A

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
4080	-	O-phospho-L-homoserine	-	Methanosarcina acetivorans C2A

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Release 2023.1 (January 2023)