BRENDA - Enzyme Database
show all sequences of 4.2.3.1

Crystal structure of threonine synthase from Arabidopsis thaliana

Thomazeau, K.; Curien, G.; Dumas, R.; Biou, V.; Protein Sci. 10, 638-648 (2001)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
S-adenosyl-L-methionine
-
Arabidopsis thaliana
Application
Application
Commentary
Organism
agriculture
as one of the few enzymes that are cross-activated by the product of another pathway, S-adenosyl-L-methionine, it has a potential application as a target for herbicides
Arabidopsis thaliana
Crystallization (Commentary)
Crystallization
Organism
hanging-drop vapour-diffusion method, crystal structure of apo threonine synthase as solved at 2.25 A resolution from triclinic crystals
Arabidopsis thaliana
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
O-phospho-L-homoserine + H2O
Arabidopsis thaliana
last reaction in the synthesis of threonine from aspartate
L-threonine + phosphate
-
Arabidopsis thaliana
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Arabidopsis thaliana
Q9S7B5
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
O-phospho-L-homoserine + H2O
-
653789
Arabidopsis thaliana
L-threonine + phosphate
-
653789
Arabidopsis thaliana
?
O-phospho-L-homoserine + H2O
last reaction in the synthesis of threonine from aspartate
653789
Arabidopsis thaliana
L-threonine + phosphate
-
653789
Arabidopsis thaliana
?
Subunits
Subunits
Commentary
Organism
dimer
four-domain dimer with a two-stranded beta-sheet arm protruding from one monomer onto the other
Arabidopsis thaliana
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
S-adenosyl-L-methionine
-
Arabidopsis thaliana
Application (protein specific)
Application
Commentary
Organism
agriculture
as one of the few enzymes that are cross-activated by the product of another pathway, S-adenosyl-L-methionine, it has a potential application as a target for herbicides
Arabidopsis thaliana
Crystallization (Commentary) (protein specific)
Crystallization
Organism
hanging-drop vapour-diffusion method, crystal structure of apo threonine synthase as solved at 2.25 A resolution from triclinic crystals
Arabidopsis thaliana
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
O-phospho-L-homoserine + H2O
Arabidopsis thaliana
last reaction in the synthesis of threonine from aspartate
L-threonine + phosphate
-
Arabidopsis thaliana
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
O-phospho-L-homoserine + H2O
-
653789
Arabidopsis thaliana
L-threonine + phosphate
-
653789
Arabidopsis thaliana
?
O-phospho-L-homoserine + H2O
last reaction in the synthesis of threonine from aspartate
653789
Arabidopsis thaliana
L-threonine + phosphate
-
653789
Arabidopsis thaliana
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
four-domain dimer with a two-stranded beta-sheet arm protruding from one monomer onto the other
Arabidopsis thaliana
Other publictions for EC 4.2.3.1
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
747483
Kaur
Evolutionary analysis of a no ...
Aquifex aeolicus, Arabidopsis thaliana, Brucella melitensis 16M, Brucella melitensis, Burkholderia thailandensis, Burkholderia thailandensis ATCC 700388, Escherichia coli, Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv, Saccharomyces cerevisiae, Thermus thermophilus
Cell Cycle
16
1918-1926
2017
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729900
Shoji
A QM/MM study of the L-threoni ...
Thermus thermophilus
J. Am. Chem. Soc.
136
4525-4533
2014
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728946
Morneau
Development of a continuous as ...
Escherichia coli
Anal. Biochem.
423
78-85
2012
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1
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1
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715628
Murakawa
Product-assisted catalysis as ...
Thermus thermophilus, Thermus thermophilus HB8 / ATCC 27634 / DSM 579
J. Biol. Chem.
286
2774-2784
2011
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1
1
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4
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4
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3
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3
3
701597
Sikdar
-
Functional analysis of a gene ...
Oryza sativa
Afr. J. Biotechnol.
9
1122-1129
2010
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1
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696127
Graham
Convergent evolution of coenzy ...
Methanosarcina acetivorans C2A
Biochem. J.
424
467-478
2009
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694038
Kingsbury
Threonine biosynthetic genes a ...
Cryptococcus neoformans
Microbiology
154
2767-2775
2008
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728848
Covarrubias
Structural, biochemical, and i ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
J. Mol. Biol.
381
622-633
2008
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1
1
1
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1
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8
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1
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1
1
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1
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665733
Mas-Droux
Allosteric threonine synthase. ...
Arabidopsis thaliana
J. Biol. Chem.
281
5188-5196
2006
1
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663091
Lee
Methionine and threonine synth ...
Escherichia coli
Plant J.
41
685-696
2005
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1
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666989
Avraham
The expression level of threon ...
Arabidopsis sp.
Transgenic Res.
14
299-311
2005
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651164
Curien
A kinetic model of the branch- ...
Arabidopsis thaliana
Eur. J. Biochem.
270
4615-4627
2003
1
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652479
Omi
Crystal structures of threonin ...
Thermus thermophilus, Thermus thermophilus HB8 / ATCC 27634 / DSM 579
J. Biol. Chem.
278
46035-46045
2003
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1
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91
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652228
Garrido-Franco
Structure and function of thre ...
Saccharomyces cerevisiae
J. Biol. Chem.
277
12396-12405
2002
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649143
Thomazeau
MAD on threonine synthase: the ...
Arabidopsis thaliana
Acta Crystallogr. Sect. D
57
1337-1340
2001
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653789
Thomazeau
Crystal structure of threonine ...
Arabidopsis thaliana
Protein Sci.
10
638-648
2001
1
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137557
Laber
Characterization of recombinan ...
Arabidopsis thaliana
Eur. J. Biochem.
263
212-221
1999
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137561
Curien
Allosteric activation of Arabi ...
Arabidopsis thaliana
Biochemistry
37
13212-13221
1998
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137562
Muhitch
-
Effects of expressing E. coli ...
Escherichia coli
Plant Physiol.
150
16-22
1997
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137555
Curien
Characterization of an Arabido ...
Arabidopsis thaliana
FEBS Lett.
390
85-90
1996
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137556
Malumbres
Analysis and expression of the ...
Corynebacterium glutamicum
Appl. Environ. Microbiol.
60
2209-2219
1994
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137560
Laber
Mechanisms of interaction of E ...
Escherichia coli
Biochemistry
33
3413-3423
1994
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137559
Farrington
Threonine synthase of Escheric ...
Escherichia coli
Arch. Biochem. Biophys.
307
165-174
1993
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3
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137558
Greenberg
Homoserine kinase and threonin ...
Glycine max
Plant Cell Rep.
7
477-480
1988
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1
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137554
Parsot
A common origin for enzymes in ...
Bacillus subtilis
Proc. Natl. Acad. Sci. USA
84
5207-5210
1987
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137552
Parsot
Evolution of biosynthetic path ...
Bacillus subtilis
EMBO J.
5
3013-3019
1986
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4
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137553
Giovanelli
Effects of orthophosphate and ...
Lemna aequinoctialis
Plant Physiol.
81
577-583
1986
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137550
Giovanelli
Threonine synthase of Lemna pa ...
Lemna aequinoctialis
Plant Physiol.
76
285-292
1984
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137551
Shames
Interaction of aspartate and a ...
Escherichia coli
J. Biol. Chem.
259
15331-15339
1984
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137549
Parsot
Nucleotide sequence of thrC an ...
Escherichia coli
Nucleic Acids Res.
11
7331-7345
1983
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137548
Thoen
-
Biosynthesis of threonine from ...
Pisum sativum
Plant Sci. Lett.
13
113-119
1978
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137547
Madison
Threonine synthetase from high ...
Beta vulgaris, Raphanus sativus
Biochem. Biophys. Res. Commun.
71
684-691
1976
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137545
Skarstedt
Threonine synthetase of Bacill ...
Bacillus subtilis
J. Biol. Chem.
248
1032-1044
1973
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137546
Schildkraut
Threonine synthetase-catalyzed ...
Bacillus subtilis
J. Bacteriol.
115
777-785
1973
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137544
Flavin
Purification and properties of ...
Neurospora crassa
J. Biol. Chem.
235
1103-1108
1960
-
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5
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