Activating Compound | Comment | Organism | Structure |
---|---|---|---|
S-adenosylmethionine | same binding site as inhibitor AMP, 85fold increase of activity, maximum activity at 0.06-0.25 mM | Arabidopsis thaliana |
Application | Comment | Organism |
---|---|---|
agriculture | possible herbicide target | Arabidopsis thaliana |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Arabidopsis thaliana |
Crystallization (Comment) | Organism |
---|---|
x-ray diffraction studies | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AMP | same binding site as S-adenosylmethionine | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
O-phosphohomoserine | in presence of S-adenosylmethionine | Arabidopsis thaliana | |
0.12 | - |
O-phosphohomoserine | without S-adenosylmethionine | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Arabidopsis thaliana | 9507 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58000 | - |
2 * 58000, comparison of nucleotide sequence and molecular mass of native enzyme | Arabidopsis thaliana |
110000 | - |
gel filtration | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
O-phospho-L-homoserine + H2O | - |
Arabidopsis thaliana | L-threonine + phosphate | - |
ir |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 58000, comparison of nucleotide sequence and molecular mass of native enzyme | Arabidopsis thaliana |
dimer | N-terminal amino acids involved in dimerization | Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
enzyme assay at | Arabidopsis thaliana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0333 | - |
O-phosphohomoserine | without S-adenosylmethionine | Arabidopsis thaliana | |
0.86 | - |
O-phosphohomoserine | in presence of S-adenosylmethionine | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
enzyme assay at | Arabidopsis thaliana |