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Literature summary for 4.2.2.9 extracted from

  • Kester, H.C.M.; Magaud, D.; Roy, C.; Anker, D.; Doutheau, A.; Shevchik, V.; Hugouvieux-Cotte-Pattat, N.; Benen, J.A.E.; Visser, J.
    Performance of selected microbial pectinases on synthetic monomethyl-esterified di- and trigalacturonates (1999), J. Biol. Chem., 274, 37053-37059.
    View publication on PubMed
Search for more literature for 4.2.2.9

Organism

Organism UniProt Comment Textmining
Aspergillus tubingensis
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-
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Dickeya chrysanthemi
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 2 different enzyme types: exopolygalacturonate lyase X, i.e. Pel X, and exopectate lyase W, i.e. PelW
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Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 substrate specificity Dickeya chrysanthemi
additional information
-
 substrate specificity Aspergillus tubingensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-methyl-di-D-galacturonate alpha-(1-4)-linked, 36% of the activity with di-D-galacturonate Aspergillus tubingensis ?
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 ?
1-methyl-tri-D-galacturonate alpha-(1-4)-linked Dickeya chrysanthemi ?
-
 ?
1-methyl-tri-D-galacturonate best substrate, alpha-(1-4)-linked Aspergillus tubingensis ?
-
 ?
2-methyl-di-D-galacturonate alpha-(1-4)-linked, 35% of the activity with di-D-galacturonate Aspergillus tubingensis ?
-
 ?
2-methyl-tri-D-galacturonate alpha-(1-4)-linked, 19% of the activity with tri-D-galacturonate or 1-methyl-tri-D-galacturonate Aspergillus tubingensis ?
-
 ?
3-methyl-tri-D-galacturonate alpha-(1-4)-linked, 18% of the activity with tri-D-galacturonate or 1-methyl-tri-D-galacturonate Aspergillus tubingensis ?
-
 ?
3-methyl-tri-D-galacturonate poor substrate only for PelW, not for PelX Dickeya chrysanthemi ?
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 ?
DELTA4,5-unsaturated tri-D-galacturonate alpha-(1-4)-linked, low activity with PelX Dickeya chrysanthemi ?
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 ?
di-D-galacturonate alpha-(1-4)-linked Aspergillus tubingensis ?
-
 ?
additional information substrate specificity, overview, no activity for both enzyme types with monomethyl-esterified digalacturonate substrates and with 2-methyl-tri-G-galacturonate Dickeya chrysanthemi ?
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 ?
tri-D-galacturonate alpha-(1-4)-linked Aspergillus tubingensis ?
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 ?
tri-D-galacturonate alpha-(1-4)-linked, best substrate for both enzyme types Dickeya chrysanthemi ?
-
 ?

Synonyms

Synonyms Comment Organism
exopectate lyase W
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 Dickeya chrysanthemi
exopolygalacturonase
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 Aspergillus tubingensis
exopolygalacturonate lyase X
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 Dickeya chrysanthemi
PelW
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 Dickeya chrysanthemi
PelX
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 Dickeya chrysanthemi

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
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 assay at Dickeya chrysanthemi

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Dickeya chrysanthemi