BRENDA - Enzyme Database
show all sequences of 4.2.2.9

Effect of chelating agents on exopolygalacturonate lyase of Erwinia carotovora subsp. carotovora

Ikeda, S.; Kegoya, Y.; Hatanaka, C.; Agric. Biol. Chem. 48, 2777-2782 (1984)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
triethylenetetramine
activates
Pectobacterium carotovorum
Inhibitors
Inhibitors
Commentary
Organism
Structure
diethylenetriaminepentaacetate
-
Pectobacterium carotovorum
Hg2+
-
Pectobacterium carotovorum
hydroxyethylenediaminetriacetate
mixed type inhibition
Pectobacterium carotovorum
trans-1,2-cyclohexanediaminetetraacetate
-
Pectobacterium carotovorum
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0013
-
polygalacturonate I
-
Pectobacterium carotovorum
0.0016
-
polygalacturonate II
-
Pectobacterium carotovorum
0.0032
-
oligogalacturonate
-
Pectobacterium carotovorum
0.0054
-
trigalacturonate
-
Pectobacterium carotovorum
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
weak activation
Pectobacterium carotovorum
Na+
-
Pectobacterium carotovorum
Organism
Organism
UniProt
Commentary
Textmining
Pectobacterium carotovorum
-
subsp. carotovora
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
oligogalacturonate
-
34119
Pectobacterium carotovorum
?
-
-
-
?
polygalacturonate
-
34119
Pectobacterium carotovorum
unsaturated digalacturonate
-
-
-
?
polygalacturonate I
-
34119
Pectobacterium carotovorum
?
-
-
-
?
polygalacturonate II
-
34119
Pectobacterium carotovorum
?
-
-
-
?
trigalacturonic acid
-
34119
Pectobacterium carotovorum
?
-
-
-
?
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
triethylenetetramine
activates
Pectobacterium carotovorum
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
diethylenetriaminepentaacetate
-
Pectobacterium carotovorum
Hg2+
-
Pectobacterium carotovorum
hydroxyethylenediaminetriacetate
mixed type inhibition
Pectobacterium carotovorum
trans-1,2-cyclohexanediaminetetraacetate
-
Pectobacterium carotovorum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0013
-
polygalacturonate I
-
Pectobacterium carotovorum
0.0016
-
polygalacturonate II
-
Pectobacterium carotovorum
0.0032
-
oligogalacturonate
-
Pectobacterium carotovorum
0.0054
-
trigalacturonate
-
Pectobacterium carotovorum
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
weak activation
Pectobacterium carotovorum
Na+
-
Pectobacterium carotovorum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
oligogalacturonate
-
34119
Pectobacterium carotovorum
?
-
-
-
?
polygalacturonate
-
34119
Pectobacterium carotovorum
unsaturated digalacturonate
-
-
-
?
polygalacturonate I
-
34119
Pectobacterium carotovorum
?
-
-
-
?
polygalacturonate II
-
34119
Pectobacterium carotovorum
?
-
-
-
?
trigalacturonic acid
-
34119
Pectobacterium carotovorum
?
-
-
-
?
Other publictions for EC 4.2.2.9
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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Hassan
PelN is a new pectate lyase of ...
Dickeya dadantii, Dickeya dadantii 3937
J. Bacteriol.
195
2197-2206
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2
1
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11
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6
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1
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1
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1
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-
1
-
-
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-
1
1
-
-
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669558
Celestino
Purification and characterizat ...
Acrophialophora nainiana
J. Biotechnol.
123
33-42
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1
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-
-
8
-
-
1
1
-
-
1
-
-
1
-
-
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2
1
3
1
1
1
-
1
1
1
-
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7
1
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8
-
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1
1
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1
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-
-
2
1
1
1
1
-
1
1
1
-
-
-
-
-
-
-
653089
Duskova
Fermentation of pectin and glu ...
Lachnospira multipara
Lett. Appl. Microbiol.
33
159-163
2001
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-
-
-
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1
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9
-
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1
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1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
34108
Shevchik
The exopolygalacturonate lyase ...
Dickeya chrysanthemi, Dickeya chrysanthemi 3937
J. Bacteriol.
181
3912-3919
1999
-
-
1
-
-
2
1
1
3
-
-
-
-
17
-
-
-
-
-
-
-
-
5
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
2
-
1
-
1
3
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
34118
Shevchik
Characterization of the exopol ...
Dickeya chrysanthemi, Dickeya chrysanthemi 3937
J. Bacteriol.
181
1652-1663
1999
-
-
1
-
-
-
1
-
-
5
1
-
-
13
-
-
1
-
-
-
1
-
12
1
-
1
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
5
1
-
-
-
-
1
-
-
1
-
12
1
1
-
1
-
1
-
-
-
-
-
-
-
-
-
651970
Kester
Performance of selected microb ...
Aspergillus tubingensis, Dickeya chrysanthemi
J. Biol. Chem.
274
37053-37059
1999
-
-
-
-
-
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
2
-
12
-
5
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
12
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
34114
Shevchik
Crystallization and preliminar ...
Dickeya chrysanthemi, Dickeya chrysanthemi 3937
Acta Crystallogr. Sect. D
54
419-422
1998
-
-
-
1
-
-
-
-
-
-
-
-
-
12
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
34119
Ikeda
-
Effect of chelating agents on ...
Pectobacterium carotovorum
Agric. Biol. Chem.
48
2777-2782
1984
1
-
-
-
-
-
4
4
-
2
-
-
-
1
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
4
-
4
-
2
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
34120
Kegoya
-
Affinity chromatography of exo ...
Pectobacterium carotovorum
Agric. Biol. Chem.
48
1055-1060
1984
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
1
-
-
-
1
-
2
1
-
-
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-
-
-
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-
-
-
-
-
-
-
-
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-
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-
1
-
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1
-
-
1
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
34115
Sato
-
Exopolygalacturonate lyase pro ...
Streptomyces massasporeus
Agric. Biol. Chem.
44
717-721
1980
-
-
-
-
-
-
1
-
-
3
1
-
-
1
-
-
1
-
-
-
-
-
5
1
-
1
-
2
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
3
1
-
-
-
-
1
-
-
-
-
5
1
1
-
2
-
1
-
1
-
-
-
-
-
-
-
34113
Sato
-
Further properties of the new ...
Streptomyces nitrosporeus
Agric. Biol. Chem.
43
1547-1551
1979
-
-
-
-
-
-
-
-
-
-
3
-
-
1
-
1
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
1
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
34117
Hatanaka
-
Effect of metal ions on activi ...
Erwinia sp.
Agric. Biol. Chem.
37
593-597
1973
-
-
-
-
-
-
3
-
-
5
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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3
-
-
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5
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
34116
Hatanaka
-
Exopectic acid transeliminase ...
Erwinia sp., Erwinia sp. W2
Agric. Biol. Chem.
36
2307-2313
1972
-
-
-
-
-
-
2
-
-
2
-
-
-
2
-
-
1
-
-
-
1
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
2
-
-
-
-
-
1
-
-
1
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
34111
Miller
Mode of action of pectic enzym ...
Clostridium multifermentans
J. Bacteriol.
102
72-78
1970
-
-
-
-
-
2
-
-
-
-
-
-
-
3
-
-
1
-
-
-
1
-
1
-
-
-
-
-
-
-
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1
-
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-
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-
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2
-
-
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-
-
-
-
-
-
-
1
-
-
1
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
34110
Macmillan
-
Exopolygalacturonate lyase fro ...
Clostridium multifermentans
Methods Enzymol.
8
632-635
1966
-
-
-
-
-
-
1
1
1
5
-
1
-
1
-
-
1
-
-
-
-
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2
-
-
-
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-
1
1
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-
1
-
1
1
5
-
1
-
-
-
1
-
-
-
-
2
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
34112
Macmillan
Purification and properties of ...
Clostridium multifermentans
Biochemistry
3
564-572
1964
-
-
-
-
-
-
-
1
-
3
-
-
-
1
-
-
1
1
-
-
-
-
2
-
-
-
-
-
-
1
1
-
-
-
-
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-
-
-
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-
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-
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1
-
3
-
-
-
-
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1
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2
-
-
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-
-
1
1
-
-
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