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Literature summary for 4.2.2.9 extracted from
- Exopolygalacturonate lyase produced by Streptomyces massasporeus (1980), Agric. Biol. Chem., 44, 717-721.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | - |
Streptomyces massasporeus |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | required, optimal concentration is 0.5 mM | Streptomyces massasporeus | |
| Mn2+ | 50% of the stimulation with Ca2+ | Streptomyces massasporeus | |
| Sr2+ | 50% of the stimulation with Ca2+ | Streptomyces massasporeus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 54000 | - |
gel filtration | Streptomyces massasporeus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces massasporeus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Streptomyces massasporeus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| hexagalacturonic acid | 51% of the activity with low molecular polygalacturonic acid | Streptomyces massasporeus | ? | - |
? | |
| pentagalacturonic acid | 60% of the activity with low molecular polygalacturonic acid | Streptomyces massasporeus | ? | - |
? | |
| polygalacturonate | low molecular polygalacturonic acid, very low activity with high molecular polygalacturonic acid | Streptomyces massasporeus | unsaturated digalacturonate | - |
? | |
| tetragalacturonic acid | 52% of the activity with low molecular polygalacturonic acid | Streptomyces massasporeus | ? | - |
? | |
| trigalacturonic acid | 248% of the activity with low molecular polygalacturonic acid | Streptomyces massasporeus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 54000, SDS-PAGE | Streptomyces massasporeus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Streptomyces massasporeus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
pH 7.0, 10 min, stable below | Streptomyces massasporeus |
| 60 | - |
pH 7.0, 10 min, 85% loss of activity | Streptomyces massasporeus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.5 | - |
- |
Streptomyces massasporeus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 10 | 2°C, 72 h, relatively stable | Streptomyces massasporeus |
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Release 2023.1 (January 2023)
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