Any feedback?
Literature summary for 4.2.2.9 extracted from
- Exopolygalacturonate lyase from Clostridium multifermentans. II. Further purification and exopolygalacturonate lyase and pectinesterase from Clostridium multifermentans (1966), Methods Enzymol., 8, 632-635.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | - |
Clostridium multifermentans |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Km for polygalacturonate is 0.114% on the basis of dry, ash-free polygalacturonic acid, in presence of 0.5 mM CaCl2 | Clostridium multifermentans |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Clostridium multifermentans | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | stimulates | Clostridium multifermentans | |
| Ca2+ | greatest stimulation of divalent cations tested, Km: 0.06 mM | Clostridium multifermentans | |
| Mg2+ | stimulates | Clostridium multifermentans | |
| Mn2+ | stimulates | Clostridium multifermentans | |
| Sr2+ | stimulates | Clostridium multifermentans | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Clostridium multifermentans | inducible | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Clostridium multifermentans | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Clostridium multifermentans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | inducible | Clostridium multifermentans | ? | - |
? | |
| polygalacturonate | - |
Clostridium multifermentans | unsaturated digalacturonate | plus one molecule of digalacturonate originating from the nonreducing end of the chain | ? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Clostridium multifermentans |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8.5 | pH 5: no activity, pH 6.0: 15% of maximal activity, pH 7.0: 40% of maximal activity, pH 8.5: maximal activity | Clostridium multifermentans |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
