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Literature summary for 4.2.2.8 extracted from

  • Ulaganathan, T.; Shi, R.; Yao, D.; Gu, R.X.; Garron, M.L.; Cherney, M.; Tieleman, D.P.; Sterner, E.; Li, G.; Li, L.; Linhardt, R.J.; Cygler, M.
    Conformational flexibility of PL12 family heparinases structure and substrate specificity of heparinase III from Bacteroides thetaiotaomicron (BT4657) (2017), Glycobiology, 27, 176-187 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.2.8

Cloned(Commentary)

Cloned (Comment) Organism
-
 Bacteroides thetaiotaomicron

Crystallization (Commentary)

Crystallization (Comment) Organism
structures of two crystal forms at resolution 1.8 and 2.4 A. Tyr301 is the main catalytic residue Bacteroides thetaiotaomicron

Protein Variants

Protein Variants Comment Organism
H431A complete loss of activity Bacteroides thetaiotaomicron
N247A complete loss of activity Bacteroides thetaiotaomicron
Y301F complete loss of activity Bacteroides thetaiotaomicron

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.593
-
 heparan sulfate pH 7.5, 23°C Bacteroides thetaiotaomicron

Organism

Organism UniProt Comment Textmining
Bacteroides thetaiotaomicron Q89YS4
-
-
Bacteroides thetaiotaomicron DSM 2079 Q89YS4
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
desulfated N-sulfated heparosan
-
 Bacteroides thetaiotaomicron desulfated N-sulfated heparosan disaccharide
-
 ?
desulfated N-sulfated heparosan
-
 Bacteroides thetaiotaomicron DSM 2079 desulfated N-sulfated heparosan disaccharide
-
 ?
heparan sulfate
-
 Bacteroides thetaiotaomicron heparan disaccharide
-
 ?
heparan sulfate
-
 Bacteroides thetaiotaomicron DSM 2079 heparan disaccharide
-
 ?
heparin
-
 Bacteroides thetaiotaomicron heparin disaccharide
-
 ?
heparin
-
 Bacteroides thetaiotaomicron DSM 2079 heparin disaccharide
-
 ?
heparosan
-
 Bacteroides thetaiotaomicron heparosan disaccharide
-
 ?
heparosan
-
 Bacteroides thetaiotaomicron DSM 2079 heparosan disaccharide
-
 ?
N-desulfated, N-acetylated heparin
-
 Bacteroides thetaiotaomicron N-desulfated, N-acetylated heparin disaccharide
-
 ?
N-desulfated, N-acetylated heparin
-
 Bacteroides thetaiotaomicron DSM 2079 N-desulfated, N-acetylated heparin disaccharide
-
 ?
N-sulfoheparosan
-
 Bacteroides thetaiotaomicron N-sulfoheüparosan disaccharide
-
 ?

Synonyms

Synonyms Comment Organism
BT4657
-
 Bacteroides thetaiotaomicron
HepIII
-
 Bacteroides thetaiotaomicron