Crystallization (Comment) | Organism |
---|---|
isoform Hep III, to 2.2 A resolution. The enzyme comprises an N-terminal alpha/alpha-barrel domain and a C-terminal antiparallel beta-sheet domain as its basic scaffold. Isoform Hep III exhibits an open form compared with the closed form of Hep II. An active site of Hep III is located in the deep cleft at the interface between its two domains | Pedobacter heparinus |
Protein Variants | Comment | Organism |
---|---|---|
E237A | 40.8% of wild-type activitiy | Pedobacter heparinus |
F423A | 42.4% of wild-type activitiy | Pedobacter heparinus |
H241A | 29.7% of wild-type activitiy | Pedobacter heparinus |
H424A | 0.3% of wild-type activitiy | Pedobacter heparinus |
I29V/L657S | 42.7% of wild-type activitiy | Pedobacter heparinus |
N240A | 3.1% of wild-type activitiy | Pedobacter heparinus |
Q238A | 88.5% of wild-type activitiy | Pedobacter heparinus |
W350A | 27.7% of wild-type activitiy | Pedobacter heparinus |
Y294F | 2.8% of wild-type activitiy | Pedobacter heparinus |
Y450F | 5.6% of wild-type activitiy | Pedobacter heparinus |
Y590 | 7.7% of wild-type activitiy | Pedobacter heparinus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pedobacter heparinus | Q59289 | - |
- |
Pedobacter heparinus DSM 2366 | Q59289 | - |
- |
Synonyms | Comment | Organism |
---|---|---|
Hep III | - |
Pedobacter heparinus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
39.4 | - |
melting temperature, presence of 1 mM heparin tetrasaccharide | Pedobacter heparinus |
40.5 | - |
melting temperature, presence of 0.1 mM heparin tetrasaccharide | Pedobacter heparinus |
43.9 | - |
melting temperature, presence of 0.1 mM heparin disaccharide | Pedobacter heparinus |
44.3 | - |
melting temperature, presence of 0.1 mM N-acetyl glucosamine | Pedobacter heparinus |
45.2 | - |
melting temperature, presence of 0.1 mM gellan tetrasaccharide | Pedobacter heparinus |
45.44 | - |
melting temperature | Pedobacter heparinus |