Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.2.2.7 extracted from

  • Balasubramaniam, K.; Sharma, K.; Rani, A.; Rajulapati, V.; Goyal, A.
    Deciphering the mode of action, structural and biochemical analysis of heparinase II/III (PsPL12a) a new member of family 12 polysaccharide lyase from Pseudopedobacter saltans (2018), Ann. Microbiol., 68, 409-418 .
No PubMed abstract available
Search for more literature for 4.2.2.7

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pseudopedobacter saltans

Inhibitors

Inhibitors Comment Organism Structure
EDTA 5 mM, about 45% residual activity Pseudopedobacter saltans
Ni2+ 2 mM, 50% residual activity Pseudopedobacter saltans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 heparin kcat/Km value 19.3 ml/mg/s, pH 6.0, 50°C Pseudopedobacter saltans

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 20 mM, 1.35fold increase in activity Pseudopedobacter saltans
Mg2+ 20 mM, 2fold increase in activity Pseudopedobacter saltans
Mn2+ 10 mM, 1.8fold increase in activity Pseudopedobacter saltans

Organism

Organism UniProt Comment Textmining
Pseudopedobacter saltans F0S8P2
-
-
Pseudopedobacter saltans DSM 12145 F0S8P2
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
heparin
-
 Pseudopedobacter saltans unsaturated heparin disaccharide + unsaturated heparin tetrasaccharide + unsaturated heparin pentasaccharide + unsaturated heparin hexasaccharide
-
 ?
heparin
-
 Pseudopedobacter saltans DSM 12145 unsaturated heparin disaccharide + unsaturated heparin tetrasaccharide + unsaturated heparin pentasaccharide + unsaturated heparin hexasaccharide
-
 ?

Subunits

Subunits Comment Organism
? x * 76530, calculated from sequence, x * 76000, SDS-PAGE Pseudopedobacter saltans

Synonyms

Synonyms Comment Organism
Pedsa_1818 locus name Pseudopedobacter saltans
PL12a
-
 Pseudopedobacter saltans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Pseudopedobacter saltans

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40
-
 90% of maximum activity Pseudopedobacter saltans
65
-
 complete loss of activity Pseudopedobacter saltans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 40
-
 Pseudopedobacter saltans
45
-
 1 h, 20% residual activity Pseudopedobacter saltans
55
-
 melting temperature Pseudopedobacter saltans
60
-
 melting temperature, presence of 10 mM Mg2+ Pseudopedobacter saltans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
8.9
-
 heparin pH 6.0, 50°C Pseudopedobacter saltans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Pseudopedobacter saltans

pH Range

pH Minimum pH Maximum Comment Organism
9
-
 complete loss of activity Pseudopedobacter saltans

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 9 stable for 1 h Pseudopedobacter saltans

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
additional information
-
 heparin Km value 4.6 mg/ml, pH 6.0, 50°C Pseudopedobacter saltans