Cloned (Comment) | Organism |
---|---|
expressed in Pedobacter heparinus | Pedobacter heparinus |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method | Pedobacter heparinus |
Protein Variants | Comment | Organism |
---|---|---|
H202/H406A | His-tagged version expressed in Escherichia coli BL21(DE3) | Pedobacter heparinus |
H202A | no activity in a real time assay, His-tagged version expressed in Escherichia coli BL21(DE3) | Pedobacter heparinus |
H202A/Y257A | His-tagged version expressed in Escherichia coli BL21(DE3) | Pedobacter heparinus |
H406A | no activity in a real time assay, His-tagged version expressed in Escherichia coli BL21(DE3) | Pedobacter heparinus |
Y257A | no activity in a real time assay, His-tagged version expressed in Escherichia coli BL21(DE3) | Pedobacter heparinus |
Y257A/H406A | His-tagged version expressed in Escherichia coli BL21(DE3) | Pedobacter heparinus |
Y257F | no activity in a real time assay, His-tagged version expressed in Escherichia coli BL21(DE3) | Pedobacter heparinus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
85000 | - |
2 * 85000, calculated: 84545 | Pedobacter heparinus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pedobacter heparinus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ion-exchange chromatography (SP-Sepharose), hydroxylapatite chromatography, mutant proteins by immobilized metal-chelate chromatography, His-tag is removed, further purification by ion-exchange chromatography | Pedobacter heparinus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | acts on heparin and heparan sulfate | Pedobacter heparinus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 85000, calculated: 84545 | Pedobacter heparinus |
Synonyms | Comment | Organism |
---|---|---|
heparin lyase II | - |
Pedobacter heparinus |
heparinase II | - |
Pedobacter heparinus |
heparitinase II | - |
Pedobacter heparinus |
HepII | - |
Pedobacter heparinus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Pedobacter heparinus | - |
- |
8.9 |