Literature summary for 4.2.2.7 extracted from

Han, Y.H.; Garron, M.L.; Kim, H.Y.; Kim, W.S.; Zhang, Z.; Ryu, K.S.; Shaya, D.; Xiao, Z.; Cheong, C.; Kim, Y.S.; Linhardt, R.J.; Jeon, Y.H.; Cygler, M.
Structural snapshots of heparin depolymerization by heparin lyase I (2009), J. Biol. Chem., 284, 34019-34027.

Search for more literature for 4.2.2.7

Cloned(Commentary)

Cloned (Comment)	Organism
His-tagged version expressed in Escherichia coli BL21(DE3)	Bacteroides thetaiotaomicron

Crystallization (Commentary)

Crystallization (Comment)	Organism
sitting drop method	Bacteroides thetaiotaomicron

Protein Variants

Protein Variants	Comment	Organism
DELTA 156-221	no activity	Bacteroides thetaiotaomicron
DELTA 191-213	about 5% of wild-type activity	Bacteroides thetaiotaomicron
H151A	no activity	Bacteroides thetaiotaomicron
K185A	about 100% of wild-type activity	Bacteroides thetaiotaomicron
K252A	about 40% of wild-type activity	Bacteroides thetaiotaomicron
K353A	no activity	Bacteroides thetaiotaomicron
Q149A	no activity	Bacteroides thetaiotaomicron
Q223A	about 40% of wild-type activity	Bacteroides thetaiotaomicron
Q22A	about 20% of wild-type activity	Bacteroides thetaiotaomicron
R156A	about 90% of wild-type activity	Bacteroides thetaiotaomicron
R83A	no activity	Bacteroides thetaiotaomicron
Y357A	no activity	Bacteroides thetaiotaomicron

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0046	-	heparin, 20-mer	wild-type protein	Bacteroides thetaiotaomicron
0.0182	-	heparin, 8-mer	wild-type protein	Bacteroides thetaiotaomicron
0.0541	-	heparin, 6-mer	wild-type protein	Bacteroides thetaiotaomicron
0.2395	-	heparin, 20-mer	deletion mutant 191-213 protein	Bacteroides thetaiotaomicron
5.015	-	heparin, 6-mer	deletion mutant 191-213 protein	Bacteroides thetaiotaomicron

Organism

Organism	UniProt	Comment	Textmining
Bacteroides thetaiotaomicron	-	-	-
Bacteroides thetaiotaomicron WAL2926	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
immobilized metal ion affinity chromatography, ion exchange chromatography, gel filtration	Bacteroides thetaiotaomicron

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
5.8	-	heparin (20-mer), deletion mutant 191-213 protein	Bacteroides thetaiotaomicron
21.1	-	heparin (6-mer), deletion mutant 191-213 protein	Bacteroides thetaiotaomicron
40.6	-	heparin (20-mer), wild-type protein	Bacteroides thetaiotaomicron
101.4	-	heparin (8-mer), wild-type protein	Bacteroides thetaiotaomicron
107.8	-	heparin (6-mer), wild-type protein	Bacteroides thetaiotaomicron

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
heparin	-	Bacteroides thetaiotaomicron	?	-	?
heparin	-	Bacteroides thetaiotaomicron WAL2926	?	-	?
heparin, 20-mer	-	Bacteroides thetaiotaomicron	unsaturated heparin disaccharides	-	?
heparin, 20-mer	-	Bacteroides thetaiotaomicron WAL2926	unsaturated heparin disaccharides	-	?
heparin, 6-mer	-	Bacteroides thetaiotaomicron	unsaturated heparin disaccharides	-	?
heparin, 6-mer	-	Bacteroides thetaiotaomicron WAL2926	unsaturated heparin disaccharides	-	?
heparin, 8-mer	-	Bacteroides thetaiotaomicron	unsaturated heparin disaccharides	-	?
heparin, 8-mer	-	Bacteroides thetaiotaomicron WAL2926	unsaturated heparin disaccharides	-	?

Synonyms

Synonyms	Comment	Organism
heparin lyase I	-	Bacteroides thetaiotaomicron
heparinase I	-	Bacteroides thetaiotaomicron

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
4	-	heparin, 20-mer	deletion mutant 191-213 protein	Bacteroides thetaiotaomicron
14.5	-	heparin, 6-mer	deletion mutant 191-213 protein	Bacteroides thetaiotaomicron
29.1	-	heparin, 20-mer	wild-type protein	Bacteroides thetaiotaomicron
73.7	-	heparin, 8-mer	wild-type protein	Bacteroides thetaiotaomicron
78.4	-	heparin, 6-mer	wild-type protein	Bacteroides thetaiotaomicron

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
3	-	heparin, 6-mer	deletion mutant 191-213 protein	Bacteroides thetaiotaomicron
16.5	-	heparin, 20-mer	deletion mutant 191-213 protein	Bacteroides thetaiotaomicron
1448	-	heparin, 6-mer	wild-type protein	Bacteroides thetaiotaomicron
4054	-	heparin, 8-mer	wild-type protein	Bacteroides thetaiotaomicron
6311	-	heparin, 20-mer	wild-type protein	Bacteroides thetaiotaomicron

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Release 2023.1 (January 2023)