Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.2.2.7 extracted from

  • Banga, J.; Tripathi, C.K.; Bihari, V.
    Growth and enzyme production kinetics of a heparinase-producing fungal isolate (2008), Med. Chem. Res., 17, 85-93.
No PubMed abstract available
Search for more literature for 4.2.2.7

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Pedobacter heparinus
-
-
extracellular the fungus produces heparinase, both extracellularly and intracellularly, in its late log phase of growth Aspergillus oryzae
-
-
intracellular
-
 Pedobacter heparinus 5622
-
intracellular the fungus produces heparinase, both extracellularly and intracellularly, in its late log phase of growth Aspergillus oryzae 5622
-

Organism

Organism UniProt Comment Textmining
Aspergillus oryzae
-
-
-
Pedobacter heparinus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
enhancement of enzyme production by 92% is achieved by manipulation of the fermentation conditions (pH: 6.5, inoculum size of 0.5-1.0%, incubation temperature 30°C) and medium ingredients (replacement of dextrose by mannitol, soy peptone by chitin and ammonium sulfate by ammonium nitrate and supplementation of pyridoxine hydrochloride and glutaric acid) Aspergillus oryzae

Source Tissue

Source Tissue Comment Organism Textmining
cell culture optimization of enzyme production, simple carbon sources, including sucrose, lactose, maltose, and complex carbon sources such as starch and cellulose are able to support growth of the organism, but enzyme can be produced only in the presence of maltose, sorbitol, mannitol, arabinose, and mannose. Phosphate inhibits enzyme production and cell growth, while heparin inhibits cell growth but is required for enzyme induction, overview. Heparinase production in optimized medium is highest, i.e., 75.4 units, when the pH of the medium is kept at pH 6.5, inoculum size of 0.5-1.0% of spore suspension, and for an incubation temperature of 30°C. Growth profile, heparin utilization, and heparinase production in optimized medium, overview Pedobacter heparinus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 heparinase activity is found to be dependent on the rate of utilization of heparin during growth. Heparin is taken up rapidly in the initial exponential phase of the growth at a rate of 0.16 g/cell/h. Enzyme activity increases at a rate of 1.53 units/h with the exhaustion of heparin in the medium Aspergillus oryzae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
heparin
-
 Aspergillus oryzae ?
-
 ?

Synonyms

Synonyms Comment Organism
Heparinase
-
 Aspergillus oryzae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Aspergillus oryzae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Aspergillus oryzae

Expression

Organism Comment Expression
Pedobacter heparinus phosphate suppresses the enzyme production in cells, enzyme production is also reduced by potassium nitrate, maltose, mannose, and especially by sucrose, overview down
Pedobacter heparinus heparin induces the enzyme in cells, enzyme production is also upleveled by addition of N-acetyl D-glucosamine, glutaric acid, mannitol, arabinose, pyridoxine, diammonium biphosphate, ammonium nitrate, and especially by chitin, slight enhancement by L-leucine, L-glutamine, and L-histidine, overview up