Activating Compound | Comment | Organism | Structure |
---|---|---|---|
EDTA | 0.1 mM, 104% increase in activity | Bacteroides stercoris | |
iodoacetic acid | 0.1 mM, 53% increase in activity | Bacteroides stercoris |
General Stability | Organism |
---|---|
enzyme is stabilized by dithiothreitol | Bacteroides stercoris |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
carbodiimide | 0.1 mM, 27% inhibition | Bacteroides stercoris | |
Cd2+ | 0.1 mM, 22% inhibition | Bacteroides stercoris | |
Mg2+ | 0.1 mM, 17% inhibition | Bacteroides stercoris | |
N-tosyl-L-lysine-chloromethyl ketone | 0.1 mM, 84% inhibition | Bacteroides stercoris | |
p-chloromercuriphenyl sulfonic acid | 0.1 mM, complete inhibition | Bacteroides stercoris |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.013 | - |
heparin | pH 7.0, 45°C, heparin from porcine mucosa | Bacteroides stercoris | |
0.21 | - |
heparan sulfate | pH 7.0, 45°C, heparin from porcine mucosa | Bacteroides stercoris |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 0.1 mM, 123% increase in activity | Bacteroides stercoris | |
Cu2+ | 0.1 mM, 14% increase in activity | Bacteroides stercoris | |
NaCl | 500 mM, required for maximal activity | Bacteroides stercoris |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48000 | - |
x * 48000, SDS-PAGE | Bacteroides stercoris |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacteroides stercoris | - |
- |
- |
Bacteroides stercoris HJ-15 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bacteroides stercoris |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
intestine | - |
Bacteroides stercoris | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-O-desulfated heparin | activity is 26% of the activity with heparin | Bacteroides stercoris | ? | - |
? | |
2-O-desulfated heparin | activity is 26% of the activity with heparin | Bacteroides stercoris HJ-15 | ? | - |
? | |
heparan sulfate | enzyme prefers heparin to heparan sulfate. Activity with heparan sulfate from porcine mucosa is 17% of the activity with heparin. Activity with heparan sulfate from bovine kidney is 15% of the activity with heparin | Bacteroides stercoris | ? | - |
? | |
heparan sulfate | enzyme prefers heparin to heparan sulfate. Activity with heparan sulfate from porcine mucosa is 17% of the activity with heparin. Activity with heparan sulfate from bovine kidney is 15% of the activity with heparin | Bacteroides stercoris HJ-15 | ? | - |
? | |
heparin | enzyme prefers heparin to heparan sulfate. The enzyme acts preferentially on the trisulfated sequence ->4-alpha-D-GlcNS6S(1->4)-alpha-L-IdoA2S1-> comprising heparin affording the unsaturated trisulfated disaccharid (DELTAUA2S-GlcNS6S) | Bacteroides stercoris | ? | - |
? | |
heparin | enzyme prefers heparin to heparan sulfate. The enzyme acts preferentially on the trisulfated sequence ->4-alpha-D-GlcNS6S(1->4)-alpha-L-IdoA2S1-> comprising heparin affording the unsaturated trisulfated disaccharid (DELTAUA2S-GlcNS6S) | Bacteroides stercoris HJ-15 | ? | - |
? | |
additional information | no activity with acharan sulfate, chondroitin sulfate and N-desulfated heparin | Bacteroides stercoris | ? | - |
? | |
additional information | no activity with acharan sulfate, chondroitin sulfate and N-desulfated heparin | Bacteroides stercoris HJ-15 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 48000, SDS-PAGE | Bacteroides stercoris |
Synonyms | Comment | Organism |
---|---|---|
heparin lyase I | - |
Bacteroides stercoris |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Bacteroides stercoris |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 56 | 30°C: about 40% of maximal activity, 56°C: about 650% of maximal activity | Bacteroides stercoris |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Bacteroides stercoris |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Bacteroides stercoris | isoelectric focusing | - |
9 |