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Literature summary for 4.2.2.7 extracted from

  • Kim, W.S.; Kim, B.T.; et.al.
    Purification of heparin lyase I from Bacteroides stercoris HJ-15 (2004), J. Biochem. Mol. Biol., 37, 684-690.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
EDTA 0.1 mM, 104% increase in activity Bacteroides stercoris
iodoacetic acid 0.1 mM, 53% increase in activity Bacteroides stercoris

General Stability

General Stability Organism
enzyme is stabilized by dithiothreitol Bacteroides stercoris

Inhibitors

Inhibitors Comment Organism Structure
carbodiimide 0.1 mM, 27% inhibition Bacteroides stercoris
Cd2+ 0.1 mM, 22% inhibition Bacteroides stercoris
Mg2+ 0.1 mM, 17% inhibition Bacteroides stercoris
N-tosyl-L-lysine-chloromethyl ketone 0.1 mM, 84% inhibition Bacteroides stercoris
p-chloromercuriphenyl sulfonic acid 0.1 mM, complete inhibition Bacteroides stercoris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.013
-
heparin pH 7.0, 45°C, heparin from porcine mucosa Bacteroides stercoris
0.21
-
heparan sulfate pH 7.0, 45°C, heparin from porcine mucosa Bacteroides stercoris

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 0.1 mM, 123% increase in activity Bacteroides stercoris
Cu2+ 0.1 mM, 14% increase in activity Bacteroides stercoris
NaCl 500 mM, required for maximal activity Bacteroides stercoris

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48000
-
x * 48000, SDS-PAGE Bacteroides stercoris

Organism

Organism UniProt Comment Textmining
Bacteroides stercoris
-
-
-
Bacteroides stercoris HJ-15
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Bacteroides stercoris

Source Tissue

Source Tissue Comment Organism Textmining
intestine
-
Bacteroides stercoris
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-O-desulfated heparin activity is 26% of the activity with heparin Bacteroides stercoris ?
-
?
2-O-desulfated heparin activity is 26% of the activity with heparin Bacteroides stercoris HJ-15 ?
-
?
heparan sulfate enzyme prefers heparin to heparan sulfate. Activity with heparan sulfate from porcine mucosa is 17% of the activity with heparin. Activity with heparan sulfate from bovine kidney is 15% of the activity with heparin Bacteroides stercoris ?
-
?
heparan sulfate enzyme prefers heparin to heparan sulfate. Activity with heparan sulfate from porcine mucosa is 17% of the activity with heparin. Activity with heparan sulfate from bovine kidney is 15% of the activity with heparin Bacteroides stercoris HJ-15 ?
-
?
heparin enzyme prefers heparin to heparan sulfate. The enzyme acts preferentially on the trisulfated sequence ->4-alpha-D-GlcNS6S(1->4)-alpha-L-IdoA2S1-> comprising heparin affording the unsaturated trisulfated disaccharid (DELTAUA2S-GlcNS6S) Bacteroides stercoris ?
-
?
heparin enzyme prefers heparin to heparan sulfate. The enzyme acts preferentially on the trisulfated sequence ->4-alpha-D-GlcNS6S(1->4)-alpha-L-IdoA2S1-> comprising heparin affording the unsaturated trisulfated disaccharid (DELTAUA2S-GlcNS6S) Bacteroides stercoris HJ-15 ?
-
?
additional information no activity with acharan sulfate, chondroitin sulfate and N-desulfated heparin Bacteroides stercoris ?
-
?
additional information no activity with acharan sulfate, chondroitin sulfate and N-desulfated heparin Bacteroides stercoris HJ-15 ?
-
?

Subunits

Subunits Comment Organism
? x * 48000, SDS-PAGE Bacteroides stercoris

Synonyms

Synonyms Comment Organism
heparin lyase I
-
Bacteroides stercoris

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Bacteroides stercoris

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 56 30°C: about 40% of maximal activity, 56°C: about 650% of maximal activity Bacteroides stercoris

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Bacteroides stercoris

pI Value

Organism Comment pI Value Maximum pI Value
Bacteroides stercoris isoelectric focusing
-
9