KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0036 | - |
heparan sulfate | pH 7.5, 40ºC | Niallia circulans | |
0.0042 | - |
heparin | pH 7.5, 40ºC | Niallia circulans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | 174% of the maximal activity at 5 mM | Niallia circulans | |
Ca2+ | 200% of the maximal activity at 5 mM | Niallia circulans | |
Co2+ | 18% of the maximal activity at 5 mM | Niallia circulans | |
Cu2+ | 3% of the maximal activity at 5 mM | Niallia circulans | |
Mg2+ | 160% of the maximal activity at 5 mM | Niallia circulans | |
Mn2+ | 51% of the maximal activity at 5 mM | Niallia circulans | |
Ni2+ | 16% of the maximal activity at 5 mM | Niallia circulans | |
Zn2+ | 13% of the maximal activity at 5 mM | Niallia circulans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
111000 | - |
x * 111000, SDS-PAGE | Niallia circulans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Niallia circulans | - |
HpT298 strain | - |
Niallia circulans HpT298 | - |
HpT298 strain | - |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation followed by DEAE-Sephacel and Sephacryl S-200 column chromatography | Niallia circulans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
heparan sulfate | - |
Niallia circulans | heparan sulfate disaccharides with 4-deoxy-alpha-D-gluc-4-enuronosyl groups at their non-reducing ends | several disaccharides formed by 4-deoxy-alpha-L-threo-hex-4-enepyranosyluronic acid and glucosamine, both free and sulfated | ? | |
heparan sulfate | - |
Niallia circulans HpT298 | heparan sulfate disaccharides with 4-deoxy-alpha-D-gluc-4-enuronosyl groups at their non-reducing ends | several disaccharides formed by 4-deoxy-alpha-L-threo-hex-4-enepyranosyluronic acid and glucosamine, both free and sulfated | ? | |
heparin | - |
Niallia circulans | unsaturated heparin disaccharide + ? | several disaccharides formed by 4-deoxy-alpha-L-threo-hex-4-enepyranosyluronic acid and glucosamine, both free and sulfated | ? | |
heparin | - |
Niallia circulans HpT298 | unsaturated heparin disaccharide + ? | several disaccharides formed by 4-deoxy-alpha-L-threo-hex-4-enepyranosyluronic acid and glucosamine, both free and sulfated | ? | |
additional information | no catalytic activity on dermatan sulfate and keratan sulfate as substrates | Niallia circulans | ? | - |
? | |
additional information | no catalytic activity on dermatan sulfate and keratan sulfate as substrates | Niallia circulans HpT298 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 111000, SDS-PAGE | Niallia circulans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
pH 7.5 | Niallia circulans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
60% of the maximal activity retained after 1h | Niallia circulans |
55 | - |
inactivation | Niallia circulans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Niallia circulans |