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Literature summary for 4.2.2.5 extracted from
- A new member of family 8 polysaccharide lyase chondroitin AC lyase (PsPL8A) from Pedobacter saltans displays endo- and exo-lytic catalysis (2016), J. Mol. Catal. B, 134, 215-224 .
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 20 mM, 2fold activation | Pseudopedobacter saltans |  |
| Na+ | 100 mM, 2fold activation | Pseudopedobacter saltans | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Pseudopedobacter saltans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudopedobacter saltans | F0S764 | - |
- |
| Pseudopedobacter saltans DSM 12145 | F0S764 | - |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 43.2 | - |
substrate hyaluronic acid, 39°C, pH 7.2 | Pseudopedobacter saltans |
| 214 | - |
substrate chondroitin 6-sulfate, 39°C, pH 7.2 | Pseudopedobacter saltans |
| 489 | - |
substrate chondroitin 4-sulfate, 39°C, pH 7.2 | Pseudopedobacter saltans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chondroitin 4-sulfate | - |
Pseudopedobacter saltans | 2-acetamido-2-deoxy-3-O-(beta-D-gluco-4-enepyranosyluronic acid)-4-O-sulfo-D-galactose | - |
? | |
| chondroitin 4-sulfate | - |
Pseudopedobacter saltans DSM 12145 | 2-acetamido-2-deoxy-3-O-(beta-D-gluco-4-enepyranosyluronic acid)-4-O-sulfo-D-galactose | - |
? | |
| chondroitin 6-sulfate | - |
Pseudopedobacter saltans | ? | - |
? | |
| chondroitin 6-sulfate | - |
Pseudopedobacter saltans DSM 12145 | ? | - |
? | |
| hyaluronic acid | - |
Pseudopedobacter saltans | ? | - |
? | |
| hyaluronic acid | - |
Pseudopedobacter saltans DSM 12145 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 77000, SDS-PAGE | Pseudopedobacter saltans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 39 | - |
- |
Pseudopedobacter saltans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
- |
Pseudopedobacter saltans |
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Release 2023.1 (January 2023)
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