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Literature summary for 4.2.2.5 extracted from

  • Williams, A.; He, W.; Cress, B.F.; Liu, X.; Alexandria, J.; Yoshizawa, H.; Nishimura, K.; Toida, T.; Koffas, M.; Linhardt, R.J.
    Cloning and expression of recombinant chondroitinase ACII and its comparison to the Arthrobacter aurescens enzyme (2017), Biotechnol. J., 12, 1700239 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.2.5

Application

Application Comment Organism
synthesis the enzyme can serve as a suitable replacement for the original enzyme, from Arthrobacter aurescens IAM 110 65, UniProt ID P84141, which is no longer commercially available Paenarthrobacter aurescens

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Paenarthrobacter aurescens

Protein Variants

Protein Variants Comment Organism
I236T mutation introduced to render the enzyme identical to ACII from Arthrobacter aurescens IAM 110 65. Mutant behaves similar to wild-type Paenarthrobacter aurescens

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Paenarthrobacter aurescens
-
-

Organism

Organism UniProt Comment Textmining
Paenarthrobacter aurescens
-
-
-
Paenarthrobacter aurescens 161MFSha2.1
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
culture fluid
-
 Paenarthrobacter aurescens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4
-
 pH 7.4, 37°C Paenarthrobacter aurescens

Storage Stability

Storage Stability Organism
-40°C, no loss by freeze-drying in presence of 100 mM lactose or trehalose, about 60% of enzymatic activity is lost on long-term storage in the absence of both trehalose and lactose Paenarthrobacter aurescens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chondroitin sulfate A
-
 Paenarthrobacter aurescens ?
-
 ?
chondroitin sulfate A
-
 Paenarthrobacter aurescens 161MFSha2.1 ?
-
 ?
chondroitin sulfate B
-
 Paenarthrobacter aurescens ?
-
 ?
chondroitin sulfate B
-
 Paenarthrobacter aurescens 161MFSha2.1 ?
-
 ?
chondroitin sulfate C
-
 Paenarthrobacter aurescens ?
-
 ?
chondroitin sulfate C
-
 Paenarthrobacter aurescens 161MFSha2.1 ?
-
 ?
chondroitin sulfate D
-
 Paenarthrobacter aurescens ?
-
 ?
chondroitin sulfate D
-
 Paenarthrobacter aurescens 161MFSha2.1 ?
-
 ?
chondroitin sulfate E
-
 Paenarthrobacter aurescens ?
-
 ?
chondroitin sulfate E
-
 Paenarthrobacter aurescens 161MFSha2.1 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 83300, calculated from sequence, x * 75000, SDS-PAGE Paenarthrobacter aurescens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Paenarthrobacter aurescens

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
50
-
 10% residual activity Paenarthrobacter aurescens

pI Value

Organism Comment pI Value Maximum pI Value
Paenarthrobacter aurescens calculated from sequence
-
 12