Protein Variants | Comment | Organism |
---|---|---|
Y234F | mutant enzyme is inactive with chondroitin 6-sulfate | Pedobacter heparinus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.6 | - |
chondroitin 6-sulfate | pH 6.8, 30°C, mutant enzyme Y234F | Pedobacter heparinus | |
0.66 | - |
chondroitin 6-sulfate | pH 6.8, 30°C, wild-type enzyme | Pedobacter heparinus | |
6.9 | - |
4-O-(2',4'-dinitrophenyl)-beta-D-glucopyranosiruronic acid | pH 6.8, 30°C, wild-type enzyme | Pedobacter heparinus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pedobacter heparinus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-O-(2',4'-dinitrophenyl)-beta-D-glucopyranosiruronic acid | - |
Pedobacter heparinus | ? | - |
? | |
chondroitin 6-sulfate | the degradation of chondroitin by an anionic E1cb elimination mechanism involves proton abstraction from C5 of glucuronic acid, Tyr234 is the key base catalysts | Pedobacter heparinus | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.041 | - |
4-O-(2',4'-dinitrophenyl)-beta-D-glucopyranosiruronic acid | pH 6.8, 30°C, wild-type enzyme | Pedobacter heparinus | |
855 | - |
chondroitin 6-sulfate | pH 6.8, 30°C, wild-type enzyme | Pedobacter heparinus |