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Literature summary for 4.2.2.5 extracted from

  • Ke, T.; Zhangfu, L.; Qing, G.; Yong, T.; Hong, J.; Hongyan, R.; Kun, L.; Shigui, L.
    Isolation of Serratia marcescens as a chondroitinase-producing bacterium and purification of a novel chondroitinase AC (2005), Biotechnol. Lett., 27, 489-493.
    View publication on PubMed
Search for more literature for 4.2.2.5

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Km-value for chondroitin sulfate A is 0.4 mg/ml, Km-value for chondroitin sulfate C is 0.5 mg/ml, at pH 7 and 30°C Serratia marcescens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
 2 * 35000, SDS-PAGE Serratia marcescens
71000
-
 gel filtration Serratia marcescens

Organism

Organism UniProt Comment Textmining
Serratia marcescens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Serratia marcescens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Serratia marcescens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chondroitin sulfate A
-
 Serratia marcescens ?
-
 ?
chondroitin sulfate C
-
 Serratia marcescens ?
-
 ?
hyaluronic acid
-
 Serratia marcescens ?
-
 ?
additional information no activity with chondroitin sulfate B, heparin or heparan sulfate Serratia marcescens ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 35000, SDS-PAGE Serratia marcescens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Serratia marcescens

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 55 25°C: about 85% of maximal activity, 55°C: about 45% of maximal activity Serratia marcescens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Serratia marcescens

pH Range

pH Minimum pH Maximum Comment Organism
5 9.5 pH 5.0: about 30% of maximal activity, pH 9.5: about 40% of maximal activity Serratia marcescens

pI Value

Organism Comment pI Value Maximum pI Value
Serratia marcescens isoelectric focusing, pH-range 3.5-10
-
 7.19