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Literature summary for 4.2.2.3 extracted from
- Biochemical characterization and degradation pattern of a novel endo-type bifunctional alginate lyase AlyA from marine bacterium Isoptericola halotolerans (2018), Mar. Drugs, 16, 258 .
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | 10 mM, 75% of initial activity | Isoptericola halotolerans |  |
| Cu2+ | 1 mM, 65% of initial activity | Isoptericola halotolerans | |
| Fe3+ | 1 mM, 88% of initial activity | Isoptericola halotolerans | |
| Ni2+ | 1 mM, 87% of initial activity | Isoptericola halotolerans | |
| Zn2+ | 1 mM, 91% of initial activity | Isoptericola halotolerans | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.2 | - |
poly(alpha-(1,4)-L-guluronic acid) | pH 7.5, 55°C | Isoptericola halotolerans | |
| 5.6 | - |
alginate | pH 7.5, 55°C | Isoptericola halotolerans | |
| 6.7 | - |
poly(beta-(1,4)-D-mannuronic acid) | pH 7.5, 55°C | Isoptericola halotolerans | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 1 mM, 174% of initial activity, 10 mM, 135% of initial activity | Isoptericola halotolerans | |
| Mg2+ | 1 mM, 168% of initial activity, 10 mM, 119% of initial activity | Isoptericola halotolerans | |
| Na+ | 100 mM, 126% of initial activity, 500 mM, 203% of initial activity | Isoptericola halotolerans | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Isoptericola halotolerans | A0A346FH09 | - |
- |
| Isoptericola halotolerans NJ-05 | A0A346FH09 | - |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 7658 | - |
substrate poly(beta-(1,4)-D-mannuronic acid), pH 7.5, 55°C | Isoptericola halotolerans |
| 7985 | - |
substrate alginate, pH 7.5, 55°C | Isoptericola halotolerans |
| 8643 | - |
substrate poly(alpha-(1,4)-L-guluronic acid), pH 7.5, 55°C | Isoptericola halotolerans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alginate | - |
Isoptericola halotolerans | unsaturated algino-oligosaccharides | - |
? | |
| alginate | - |
Isoptericola halotolerans NJ-05 | unsaturated algino-oligosaccharides | - |
? | |
| poly(alpha-(1,4)-L-guluronic acid) | reaction of EC 4.2.2.11 | Isoptericola halotolerans | 4-O-(4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl)-(1,4)-alpha-oligo(alpha-(1,4)-L-guluronate) | - |
? | |
| poly(alpha-(1,4)-L-guluronic acid) | reaction of EC 4.2.2.11 | Isoptericola halotolerans NJ-05 | 4-O-(4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl)-(1,4)-alpha-oligo(alpha-(1,4)-L-guluronate) | - |
? | |
| poly(beta-(1,4)-D-mannuronic acid) | - |
Isoptericola halotolerans | 4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl-(1,4)-beta-oligo(beta-(1,4)-D-mannuronate) | - |
? | |
| poly(beta-(1,4)-D-mannuronic acid) | - |
Isoptericola halotolerans NJ-05 | 4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl-(1,4)-beta-oligo(beta-(1,4)-D-mannuronate) | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 25320, calculated from sequence, including His-tag | Isoptericola halotolerans |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AlyA | - |
Isoptericola halotolerans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
- |
Isoptericola halotolerans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
30 min, 80% residual activity | Isoptericola halotolerans |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 24.8 | - |
poly(alpha-(1,4)-L-guluronic acid) | pH 7.5, 55°C | Isoptericola halotolerans | |
| 26.9 | - |
poly(beta-(1,4)-D-mannuronic acid) | pH 7.5, 55°C | Isoptericola halotolerans | |
| 45.9 | - |
alginate | pH 7.5, 55°C | Isoptericola halotolerans | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Isoptericola halotolerans |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 9 | 24 h, more than 60% resiudal activity | Isoptericola halotolerans |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4 | - |
poly(beta-(1,4)-D-mannuronic acid) | pH 7.5, 55°C | Isoptericola halotolerans | |
| 7.6 | - |
poly(alpha-(1,4)-L-guluronic acid) | pH 7.5, 55°C | Isoptericola halotolerans | |
| 8.2 | - |
alginate | pH 7.5, 55°C | Isoptericola halotolerans | |
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