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Literature summary for 4.2.2.3 extracted from

  • Zhu, B.W.; Huang, L.S.; Tan, H.D.; Qin, Y.Q.; Du, Y.G.; Yin, H.
    Characterization of a new endo-type polyM-specific alginate lyase from Pseudomonas sp. (2015), Biotechnol. Lett., 37, 409-415 .
    View publication on PubMed
Search for more literature for 4.2.2.3

Activating Compound

Activating Compound Comment Organism Structure
PMSF 1 mm, 126% of initial activity Pseudomonas sp. E03

Inhibitors

Inhibitors Comment Organism Structure
Co2+ 1 mM, 59% of initial activity Pseudomonas sp. E03
Cu2+ 1 mM, 84% of initial activity Pseudomonas sp. E03
EDTA 1 mM, 75% of initial activity Pseudomonas sp. E03
Fe2+ 1 mM, 68% of initial activity Pseudomonas sp. E03
Mn2+ 1 mM, 32% of initial activity Pseudomonas sp. E03
SDS 1mM, 88% of initial activity Pseudomonas sp. E03
TritonX-100 1 mM, 83% of initial activity Pseudomonas sp. E03
Tween 20 1 mM, 75% of initial activity Pseudomonas sp. E03
Urea 1 mM, 86% of initial activity Pseudomonas sp. E03

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ 1 mM, 116% of initial activity Pseudomonas sp. E03
Ca2+ 1 mM, 116% of initial activity Pseudomonas sp. E03
K+ 1 mM, 121% of initial activity Pseudomonas sp. E03
Mg2+ 1 mM, 126% of initial activity Pseudomonas sp. E03
Na+ 1 mM, 120% of initial activity Pseudomonas sp. E03
Zn2+ 1 mM, 114% of initial activity Pseudomonas sp. E03

Organism

Organism UniProt Comment Textmining
Pseudomonas sp. E03 A0A0A1DZ36
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein, purified using Ni-NTA Sepharose Pseudomonas sp. E03

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
222
-
 pH 8.0, 30°C Pseudomonas sp. E03

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alginate
-
 Pseudomonas sp. E03 unsaturated algino-oligosaccharides
-
 ?
additional information enzyme mainly and endolytically depolymerizes polymannuronate by beta-elimination into oligosaccharides with degrees of polymerization of 2-5 Pseudomonas sp. E03 ?
-
 ?
poly(alpha-(1,4)-L-guluronate)
-
 Pseudomonas sp. E03 4-O-(4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl)-(1,4)-alpha-oligo(alpha-(1,4)-L-guluronate)
-
 ?
poly(beta-(1,4)-D-mannuronate)
-
 Pseudomonas sp. E03 4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl-(1,4)-beta-oligo(beta-(1,4)-D-mannuronate)
-
 ?

Subunits

Subunits Comment Organism
? x * 40400, calculated from sequence, x * 40000, SDS-PAGE, recombinant His-tagged protein Pseudomonas sp. E03

Synonyms

Synonyms Comment Organism
AlgA
-
 Pseudomonas sp. E03

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Pseudomonas sp. E03

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 stable below Pseudomonas sp. E03

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Pseudomonas sp. E03

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 9
-
 Pseudomonas sp. E03