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Literature summary for 4.2.2.3 extracted from

  • Wang, Y.; Guo, E.W.; Yu, W.G.; Han, F.
    Purification and characterization of a new alginate lyase from a marine bacterium Vibrio sp. (2013), Biotechnol. Lett., 35, 703-708.
    View publication on PubMed
Search for more literature for 4.2.2.3

Inhibitors

Inhibitors Comment Organism Structure
AlCl3 53% inhibition at 1 mM Vibrio sp.
BaCl2 28% inhibition at 1 mM Vibrio sp.
CuCl2 97% inhibition at 1 mM Vibrio sp.
EDTA 96% inhibition at 1 mM Vibrio sp.
FeCl3 92% inhibition at 1 mM Vibrio sp.
NiCl2 33% inhibition at 1 mM Vibrio sp.
ZnCl2 58% inhibition at 1 mM Vibrio sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Vibrio sp.
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
CaCl2 20% activation at 1 mM Vibrio sp.
MgCl2 15% activation at 1 mM Vibrio sp.
MnCl2 12% activation at 1 mM Vibrio sp.
additional information no effect on enzyme activity by SDS, K+, Li+, and NH4+ Vibrio sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
 x * 37000, SDS-PAGE Vibrio sp.

Organism

Organism UniProt Comment Textmining
Vibrio sp.
-
-
-
Vibrio sp. QY105
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native extracellular enzyme 23.6fold from cell culture supernatant Vibrio sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2152
-
 purified enzyme, pH 7.0, 38°C Vibrio sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information AlyV5 shows activities towards both polyguluronate and polymannuronate, but degrades the former more efficiently. AlyV5 mainly produces disaccharide, trisaccharide and tetrasaccharide from polyguluronate, trisaccharide, tetrasaccharide and pentasaccharide from polymannuronate Vibrio sp. ?
-
 ?
additional information AlyV5 shows activities towards both polyguluronate and polymannuronate, but degrades the former more efficiently. AlyV5 mainly produces disaccharide, trisaccharide and tetrasaccharide from polyguluronate, trisaccharide, tetrasaccharide and pentasaccharide from polymannuronate Vibrio sp. QY105 ?
-
 ?
poly(beta-D-mannuronate) about 30% activity compared to alginate Vibrio sp. ?
-
 ?
poly(beta-D-mannuronate) about 30% activity compared to alginate Vibrio sp. QY105 ?
-
 ?
sodium alginate high activity Vibrio sp. ?
-
 ?
sodium alginate high activity Vibrio sp. QY105 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 37000, SDS-PAGE Vibrio sp.

Synonyms

Synonyms Comment Organism
alginate lyase
-
 Vibrio sp.
AlyV5
-
 Vibrio sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
38
-
-
 Vibrio sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
90
-
 purified native enzyme, 10 min, pH 7.0, 40% activity remians Vibrio sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Vibrio sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 9 purified native enzyme, 40°C, stable within this pH range Vibrio sp.