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Literature summary for 4.2.2.3 extracted from
- Comparative study on general properties of alginate lyases from some marine gastropod mollusks (2009), Fish. Sci., 75, 755-763.
No PubMed abstract available
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 28000 | - |
SDS-PAGE | Haliotis discus hannai |
| 28000 | - |
three alginate lyases with molecular masses of 35000, 32000, and 28000 Da are isolated, SDS-PAGE | Littorina brevicula |
| 32000 | - |
three alginate lyases with molecular masses of 35000, 32000, and 28000 Da are isolated, SDS-PAGE | Littorina brevicula |
| 34000 | - |
SDS-PAGE | Haliotis iris |
| 34000 | - |
SDS-PAGE | Omphalius rusticus |
| 35000 | - |
three alginate lyases with molecular masses of 35000, 32000, and 28000 Da are isolated, SDS-PAGE | Littorina brevicula |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Haliotis discus hannai | - |
- |
- |
| Haliotis iris | - |
- |
- |
| Littorina brevicula | - |
- |
- |
| Omphalius rusticus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate fractionation, Toyopearl CM-650 M column chromatography, and hydroxyapatite column chromatography | Haliotis discus hannai |
| ammonium sulfate fractionation, Toyopearl CM-650 M column chromatography, and hydroxyapatite column chromatography | Littorina brevicula |
| ammonium sulfate fractionation, Toyopearl CM-650 M column chromatography, and hydroxyapatite column chromatography | Haliotis iris |
| ammonium sulfate fractionation, Toyopearl CM-650 M column chromatography, and hydroxyapatite column chromatography | Omphalius rusticus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| hepatopancreas | - |
Haliotis discus hannai | - |
| hepatopancreas | - |
Littorina brevicula | - |
| hepatopancreas | - |
Haliotis iris | - |
| hepatopancreas | - |
Omphalius rusticus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alginate | the enzyme preferably degrades poly-beta-D-mannuronic acid-rich substrate, is also able to degrade poly-beta-D-mannuronic acid/poly-alpha-L-guluronic acid block but hardly poly-alpha-L-guluronic acid block | Haliotis discus hannai | unsaturated algino-oligosaccharides | - |
? |  |
| alginate | the enzyme preferably degrades poly-beta-D-mannuronic acid-rich substrate, is also able to degrade poly-beta-D-mannuronic acid/poly-alpha-L-guluronic acid block but hardly poly-alpha-L-guluronic acid block | Littorina brevicula | unsaturated algino-oligosaccharides | - |
? | |
| alginate | the enzyme preferably degrades poly-beta-D-mannuronic acid-rich substrate, is also able to degrade poly-beta-D-mannuronic acid/poly-alpha-L-guluronic acid block but hardly poly-alpha-L-guluronic acid block | Haliotis iris | unsaturated algino-oligosaccharides | - |
? | |
| alginate | the enzyme preferably degrades poly-beta-D-mannuronic acid-rich substrate, is also able to degrade poly-beta-D-mannuronic acid/poly-alpha-L-guluronic acid block but hardly poly-alpha-L-guluronic acid block | Omphalius rusticus | unsaturated algino-oligosaccharides | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alginate lyase | - |
Haliotis discus hannai |
| alginate lyase | - |
Littorina brevicula |
| alginate lyase | - |
Haliotis iris |
| alginate lyase | - |
Omphalius rusticus |
| ALY | - |
Haliotis discus hannai |
| ALY | - |
Haliotis iris |
| ALY | - |
Omphalius rusticus |
| Aly28 | isoform | Littorina brevicula |
| Aly32 | isoform | Littorina brevicula |
| Aly35 | isoform | Littorina brevicula |
| poly(M) lyase | - |
Haliotis discus hannai |
| poly(M) lyase | - |
Littorina brevicula |
| poly(M) lyase | - |
Haliotis iris |
| poly(M) lyase | - |
Omphalius rusticus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
- |
Haliotis iris |
| 45 | - |
- |
Haliotis discus hannai |
| 45 | - |
- |
Omphalius rusticus |
| 50 | - |
- |
Littorina brevicula |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 38 | - |
temperature at which activity decreases to 50% of the original activity for 20 min incubation is 38°C | Haliotis iris |
| 41 | - |
temperature at which activity decreases to 50% of the original activity for 20 min incubation is 43°C | Omphalius rusticus |
| 43 | - |
temperature at which activity decreases to 50% of the original activity for 20 min incubation is 41°C | Haliotis discus hannai |
| 50 | - |
temperature at which activity decreases to 50% of the original activity for 20 min incubation is 50°C | Littorina brevicula |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Littorina brevicula |
| 8 | 8.5 | - |
Haliotis discus hannai |
| 8 | 8.5 | - |
Haliotis iris |
| 8 | 8.5 | - |
Omphalius rusticus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 11 | the pH range at which 90% or more activity of the enzymes remains s pH 3.0-11.0 | Littorina brevicula |
| 6 | 9 | the pH range at which 90% or more activity of the enzymes remained was pH 6.0-9.0 | Haliotis discus hannai |
| 6 | 9 | the pH range at which 90% or more activity of the enzymes remains | Haliotis iris |
| 6 | 9 | the pH range at which 90% or more activity of the enzymes remains | Omphalius rusticus |
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Release 2023.1 (January 2023)
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