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Literature summary for 4.2.2.3 extracted from

  • Volpi, N.
    Micellar electrokinetic capillary chromatography determination of alginic acid in pharmaceutical formulations after treatment with alginate lyase and UV detection (2008), Electrophoresis, 29, 3504-3510.
    View publication on PubMed

Application

Application Comment Organism
biotechnology a new highly specific and sensitive capillary electrophoresis method for the determination of the total alginic acid (AA) content in pharmaceutical formulations is described by means of capillary electrophoresis at 230 nm after treatment with alginate lyase and separation of unsaturated products, DELTA-oligomers (DELTA-HexA-[HexA]n), in particular, DP3 (DELTA-HexA-HexA-HexA) and DP4 (DELTAHexA-HexA-HexA-HexA). The capillary electrophoresis method is applied to the determination of AA content of both solid and liquid formulations that also contain antacid ingredients, mainly aluminium, sodium and potassium bicarbonate, and emulsifying and flavouring agents Flavobacterium sp.

Organism

Organism UniProt Comment Textmining
Flavobacterium sp.
-
-
-

Synonyms

Synonyms Comment Organism
alginate lyase
-
Flavobacterium sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Flavobacterium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Flavobacterium sp.