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Literature summary for 4.2.2.26 extracted from
- Cloning, expression, and biochemical characterization of two new oligoalginate lyases with synergistic degradation capability (2018), Mar. Biotechnol., 20, 75-86 .
Application
| Application | Comment | Organism |
|---|---|---|
| degradation | combining exotype alginate lyases OalC6 and OalC17 and the endotype alginate lyase AlySY08 enables the production of alginate monomers due to synergistic processes | Cellulophaga sp. SY116 |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Cellulophaga sp. SY116 |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Al3+ | 1 mM, 35% of initial activity; 1 mM, 62% of initial activity | Cellulophaga sp. SY116 |  |
| Cu2+ | 1 mM, 40% of initial activity; 1 mM, 54% of initial activity | Cellulophaga sp. SY116 | |
| EDTA | 1 mM, 22% of initial activity; 1 mM, 43% of initial activity | Cellulophaga sp. SY116 | |
| Fe3+ | 1 mM, 24% of initial activity; 1 mM, 36% of initial activity | Cellulophaga sp. SY116 | |
| K+ | 1 mM, 72% of initial activity | Cellulophaga sp. SY116 | |
| Mn2+ | 1 mM, 63% of initial activity; 1 mM, 63% of initial activity | Cellulophaga sp. SY116 | |
| Ni2+ | 1 mM, 57% of initial activity; 1 mM, 66% of initial activity | Cellulophaga sp. SY116 | |
| SDS | 1 mM, 62% of initial activity; 1 mM, 68% of initial activity | Cellulophaga sp. SY116 | |
| Zn2+ | 1 mM, 48% of initial activity; 1 mM, 61% of initial activity | Cellulophaga sp. SY116 | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
alginate | Km value 0.79 mg/ml, pH 7.0, 40°C | Cellulophaga sp. SY116 | |
| additional information | - |
alginate | Km value 0.95 mg/ml, pH 7.0, 40°C | Cellulophaga sp. SY116 | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 10 mM, 117% of initial activity | Cellulophaga sp. SY116 | |
| Ca2+ | 10 mM, 127% of initial activity | Cellulophaga sp. SY116 | |
| K+ | 1 mM, 115% of initial activity | Cellulophaga sp. SY116 | |
| Na+ | 10 mM, 142% of initial activity | Cellulophaga sp. SY116 | |
| Na+ | 10 mM, 151% of initial activity | Cellulophaga sp. SY116 | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cellulophaga sp. SY116 | A0A345ANR9 | - |
- |
| Cellulophaga sp. SY116 | A0A345ANS0 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant proteon | Cellulophaga sp. SY116 |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 12.6 | - |
substrate poly(beta-1,4-D-mannuronate), pH 7.0, 40°C | Cellulophaga sp. SY116 |
| 33.7 | - |
substrate alginate, pH 7.0, 40°C | Cellulophaga sp. SY116 |
| 76.8 | - |
substrate poly(alpha-1,4-L-guluronate), pH 7.0, 40°C | Cellulophaga sp. SY116 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alginate | - |
Cellulophaga sp. SY116 | unsaturated algino-monosaccharides | - |
? | |
| poly(alpha-1,4-L-guluronate) | - |
Cellulophaga sp. SY116 | ? | - |
? | |
| poly(beta-1,4-D-mannuronate) | - |
Cellulophaga sp. SY116 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 85700, calculated from sequence, x * 85000, SDS-PAGE | Cellulophaga sp. SY116 |
| ? | x * 85900, calculated from sequence, x * 85000, SDS-PAGE | Cellulophaga sp. SY116 |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| OalC17 | - |
Cellulophaga sp. SY116 |
| OalC6 | - |
Cellulophaga sp. SY116 |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Cellulophaga sp. SY116 |
| 45 | - |
- |
Cellulophaga sp. SY116 |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
1 h, 55% residual activity | Cellulophaga sp. SY116 |
| 40 | - |
1 h, 20% residual activity | Cellulophaga sp. SY116 |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.6 | - |
- |
Cellulophaga sp. SY116 |
| 7.8 | - |
- |
Cellulophaga sp. SY116 |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8 | 6 h, 4°C, more than 80% residual activity | Cellulophaga sp. SY116 |
| 6 | 8 | 6 h, 4°C, more than 85% residual activity | Cellulophaga sp. SY116 |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Cellulophaga sp. SY116 | calculated from sequence | - |
8.3 |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | enzyme degrades both alginate polymers and oligomers into monomers in an exolytic mode. Isoform OalC6 prefers alpha-L-guluronate blocks, while isoform OalC17 prefers poly beta-D-mannuronate blocks. The combination of OalC6 and OalC17 shows synergistic degradation ability toward both alginate polymers and oligomers | Cellulophaga sp. SY116 |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
alginate | kcat/Km value 114.4 ml/mg/s, pH 7.0, 40°C | Cellulophaga sp. SY116 | |
| additional information | - |
alginate | kcat/Km value 118.8 ml/mg/s, pH 7.0, 40°C | Cellulophaga sp. SY116 | |
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