Activating Compound | Comment | Organism | Structure |
---|---|---|---|
CaCl2 | 1 mM, 1.4fold activation | Geobacillus stearothermophilus | |
FeCl2 | 1 mM, 1.2fold activation | Geobacillus stearothermophilus | |
KCl | 1 mM, 1.4fold activation | Geobacillus stearothermophilus | |
Li2SO4 | 1 mM, 1.3fold activation | Geobacillus stearothermophilus | |
MgCl2 | 1 mM, 1.4fold activation | Geobacillus stearothermophilus | |
MnCl2 | 1 mM, 1.4fold activation | Geobacillus stearothermophilus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 1 mM, 50% inhibition | Geobacillus stearothermophilus | |
EDTA | 1 mM, 22% inhibition | Geobacillus stearothermophilus | |
N-bromosuccinimide | 1 mM, 81% inhibition | Geobacillus stearothermophilus | |
p-chloromercuribenzoate | 1 mM, 39% inhibition | Geobacillus stearothermophilus | |
sodium lauryl sulfate | 1 mM, 81% inhibition | Geobacillus stearothermophilus | |
Urea | 1 mM, 22% inhibition | Geobacillus stearothermophilus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | the strain produces thermostable gellan lyase extracellularly during exponential phase | Geobacillus stearothermophilus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
120000 | - |
3 * or 4 * 120000, SDS-PAGE | Geobacillus stearothermophilus |
350000 | - |
two protein bands are observed: 350000 Da and 480000 Da, non-denaturing gel electrophoresis | Geobacillus stearothermophilus |
480000 | - |
two protein bands are observed: 350000 Da and 480000 Da, non-denaturing gel electrophoresis | Geobacillus stearothermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | P85513 | isolated from Bulgarian hot spring | - |
Geobacillus stearothermophilus 98 | P85513 | isolated from Bulgarian hot spring | - |
Purification (Comment) | Organism |
---|---|
- |
Geobacillus stearothermophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.99 | - |
pH 7.0, 70°C | Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
gellan | - |
Geobacillus stearothermophilus | ? | - |
? | |
gellan | - |
Geobacillus stearothermophilus 98 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | 3 * or 4 * 120000, SDS-PAGE | Geobacillus stearothermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Geobacillus stearothermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
24 h, stable | Geobacillus stearothermophilus |
70 | - |
half-life: 50 min in absence of substrate. Stable for 2.5 h in presence of substrate | Geobacillus stearothermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | - |
Geobacillus stearothermophilus |
Organism | Comment | Expression |
---|---|---|
Geobacillus stearothermophilus | enzyme activity increases tenfold in conditions of continuous cultivation compared to data from batch fermentations and enzyme productivity is almost sixfold higher | up |